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Titolo:
Differential secretion of Fas ligand- or APO2 ligand/TNF-related apoptosis-inducing ligand-carrying microvesicles during activation-induced death of human T cells
Autore:
Monleon, I; Martinez-Lorenzo, MJ; Monteagudo, L; Lasierra, P; Taules, M; Iturralde, M; Pineiro, A; Larrad, L; Alava, MA; Naval, J; Anel, A;
Indirizzi:
Univ Zaragoza, Fac Ciencias, Dept Bioquim & Biol Mol & Celular, E-50009 Zaragoza, Spain Univ Zaragoza Zaragoza Spain E-50009 & Celular, E-50009 Zaragoza, Spain Univ Zaragoza, Hosp Clin Univ, Serv Inmunol, E-50009 Zaragoza, Spain Univ Zaragoza Zaragoza Spain E-50009 rv Inmunol, E-50009 Zaragoza, Spain Univ Zaragoza, Fac Vet, Dept Anat Embriol & Genet, E-50009 Zaragoza, SpainUniv Zaragoza Zaragoza Spain E-50009 ol & Genet, E-50009 Zaragoza, Spain Univ Barcelona, Fac Med, Serv Cient Tecn, Barcelona 7, Spain Univ Barcelona Barcelona Spain 7 ed, Serv Cient Tecn, Barcelona 7, Spain
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 12, volume: 167, anno: 2001,
pagine: 6736 - 6744
SICI:
0022-1767(200112)167:12<6736:DSOFLO>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; SOLUBLE FORM; MEDIATED CYTOTOXICITY; LYMPHOCYTES; EXPRESSION; RELEASE; INDUCTION; GRANULES; EXOSOMES; PERFORIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Anel, A Univ Zaragoza, Fac Ciencias, Dept Bioquim & Biol Mol & Celular, E-50009 Zaragoza, Spain Univ Zaragoza Zaragoza Spain E-50009 ar, E-50009 Zaragoza, Spain
Citazione:
I. Monleon et al., "Differential secretion of Fas ligand- or APO2 ligand/TNF-related apoptosis-inducing ligand-carrying microvesicles during activation-induced death of human T cells", J IMMUNOL, 167(12), 2001, pp. 6736-6744

Abstract

Preformed Fas ligand (FasL) and APO2 ligand (APO2L)/TNF-related apoptosis-inducing ligand (TRAIL) are stored in the cytoplasm of the human Jurkat T cell line and of normal human T cell blasts. The rapid release of these molecules in their bioactive form is involved in activation-induced cell death. In this study, we show by confocal microscopy that FasL and APO2L/TRAIL are mainly localized in lysosomal-like compartments in these cells. We show also by immunoelectron microscopy that FasL and APO2L/TRAIL are stored inside cytoplasmic compartments similar to 500 nm in diameter, with characteristics of multivesicular bodies. Most of these compartments share FasL and APO2L/TRAIL, although exclusive APO2L/TRAIL labeling can be also observed in separate compartments. Upon PRA activation, the mobilization of these compartments toward the plasma membrane is evident, resulting in the secretion ofthe internal microvesicles loaded with FasL and APO2L/TRAIL. In the case of activation with anti-CD59 mAb, the secretion of microvesicles labeled preferentially with APO2L/TRAIL predominates. These data provide the basis of a new and efficient mechanism for the rapid induction of autocrine or paracrine cell death during immune regulation and could modify the interpretation of the role of FasL and APO2L/TRAIL as effector mechanisms in physiological and pathological situations.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/10/20 alle ore 00:23:20