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Titolo:
Integration of the rolA gene into the genome of the vigorous apple rootstock A2 reduced plant height and shortened internodes
Autore:
Zhu, LH; Ahlman, A; Li, XY; Welander, M;
Indirizzi:
Swedish Univ Agr Sci, Dept Crop Sci, S-23053 Alnarp, Sweden Swedish Univ Agr Sci Alnarp Sweden S-23053 p Sci, S-23053 Alnarp, Sweden
Titolo Testata:
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
fascicolo: 6, volume: 76, anno: 2001,
pagine: 758 - 763
SICI:
1462-0316(200111)76:6<758:IOTRGI>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSGENIC TOBACCO PLANTS; ADVENTITIOUS SHOOT REGENERATION; RHIZOGENES T-DNA; AGROBACTERIUM-RHIZOGENES; NUCLEOTIDE-SEQUENCE; TRANSFORMATION; SENSITIVITY; EXPRESSION; PLASMID; GROWTH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Zhu, LH Swedish Univ Agr Sci, Dept Crop Sci, Box 44, S-23053 Alnarp, Sweden Swedish Univ Agr Sci Box 44 Alnarp Sweden S-23053 Alnarp, Sweden
Citazione:
L.H. Zhu et al., "Integration of the rolA gene into the genome of the vigorous apple rootstock A2 reduced plant height and shortened internodes", J HORT SCI, 76(6), 2001, pp. 758-763

Abstract

The aim of this work was to dwarf the vigorous apple rootstock A2 by insertion of the rolA gene. To optimize conditions for a successful transformation, regeneration tests were carried out. The use of sucrose in the regeneration medium gave higher regeneration frequency than sorbitol in some cases and the shoot number per regenerated leaf was higher at 10 muM TDZ comparedwith 2.5 muM TDZ on the sucrose medium. Two transgenic clones, verified byPCR and Southern analysis, have been obtained on the sucrose medium together with 2.5 muM TDZ and 1.0 or 2.5 muM NAA and wounding by forceps. The twoclones, named LA1 and LA2, contained both the rolA and nptII genes. The results of in vitro rooting showed that LA1 had a lower rooting percentage and a reduced root number per rooted shoot than the untransformed control shoots and the clone LA2 on the rooting medium containing 5 muM IBA. Growth analysis revealed that both transgenic clones had a reduced plant height and a shortened internode length compared with the control plants. However, thenode number and the stem diameter were significantly larger for clone LA1 than clone LA2 and the control plants.

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Documento generato il 03/04/20 alle ore 08:59:31