Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Somatic embryogenesis and embryo encapsulation in Fraxinus angustifolia Vhal.
Autore:
Tonon, G; Capuana, M; Rossi, C;
Indirizzi:
Univ Bologna, Dipartimento Colture Arboree, I-40126 Bologna, Italy Univ Bologna Bologna Italy I-40126 lture Arboree, I-40126 Bologna, Italy CNR, Ist Miglioramento Genet Piante Forestali, I-50134 Florence, Italy CNR Florence Italy I-50134 net Piante Forestali, I-50134 Florence, Italy
Titolo Testata:
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
fascicolo: 6, volume: 76, anno: 2001,
pagine: 753 - 757
SICI:
1462-0316(200111)76:6<753:SEAEEI>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALLUS PRODUCTION; WHITE ASH; WALNUT; REGENERATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Tonon, G Univ Bologna, Dipartimento Colture Arboree, Via F Re 6, I-40126 Bologna, Italy Univ Bologna Via F Re 6 Bologna Italy I-40126 126 Bologna, Italy
Citazione:
G. Tonon et al., "Somatic embryogenesis and embryo encapsulation in Fraxinus angustifolia Vhal.", J HORT SCI, 76(6), 2001, pp. 753-757

Abstract

This is the first paper on somatic embryogenesis in F. angustifolia, an important tree in the Mediterranean Basin for landscaping and wood production. The induction of somatic embryogenesis from immature zygotic embyros and the subsequent development, maturation, encapsulation and conversion of somatic embryos into plantlets, as well as the long-term maintenance of embryogenic capacity, are reported. The developmental stage of immature zygotic embryos affected morphogenic response. An auxin-cytokinin ratio of 2:1 (2,4-dichlorophenoxyacetic acid: benzyladenine) was essential for induction. A combination of 2,4-dichlorphenoxyacetic acid (0.44 muM) and benzyladenine (4.4 muM) has made it possible to maintain embryogenic capacity for over three years. Mannitol (30 g l(-1)) and activated charcoal (2 g l(-1)) positively affected somatic embryo maturation. The encapsulation of somatic embryos in sodium-alginate beads adversely affected plant conversion, regardless ofnutrient additions to the alginate. Sixty-five per cent of nonencapsulatedsomatic embryos were successfully converted into whole plantlets on hormone-free MS medium in comparison to 23% of the encapsulated ones.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 07:43:11