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Titolo:
Localization and characterization of NPY/PYY receptors in rat frontoparietal cortex during development
Autore:
Leroux, P;
Indirizzi:
Univ Rouen, Lab DIFEMA, UPRES EA 2122, Fac Med & Pharm, F-76183 Rouen, France Univ Rouen Rouen France F-76183 , Fac Med & Pharm, F-76183 Rouen, France
Titolo Testata:
JOURNAL OF COMPARATIVE NEUROLOGY
fascicolo: 1, volume: 442, anno: 2002,
pagine: 35 - 47
SICI:
0021-9967(20020101)442:1<35:LACONR>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; NEUROPEPTIDE-Y Y-1; PANCREATIC-POLYPEPTIDE RECEPTOR; IMMUNOREACTIVE NEURONS; MESSENGER-RNA; PEPTIDE YY; AUTORADIOGRAPHIC DISTRIBUTION; IMMUNOHISTOCHEMICAL ANALYSIS; DIFFERENTIAL DISTRIBUTION; SOMATOSTATIN RECEPTORS;
Keywords:
autoradiography; ontogeny; receptor binding; NPY13-36; [Len(31),Pro(34)]Npy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Leroux, P Univ Rouen, Lab DIFEMA, UPRES EA 2122, Fac Med & Pharm, 22 Blvd Gambetta, F-76183 Rouen, France Univ Rouen 22 Blvd Gambetta Rouen France F-76183 Rouen, France
Citazione:
P. Leroux, "Localization and characterization of NPY/PYY receptors in rat frontoparietal cortex during development", J COMP NEUR, 442(1), 2002, pp. 35-47

Abstract

Neuropeptide Y(NPY) is present in most cerebrocortical areas during fetal and postnatal development. In the rat frontal cortex, a dense radial fiber network containing NPY immunoreactivity is observed transiently as early asembryonic day 17 (E17) and disappears at the end of the first postnatal week. We have investigated the distribution of NPY receptors in the frontoparietal cortex at 13 stages of development, from E15 fetuses to adults, by invitro autoradiography, using I-125-pPYY as a radioligand. Quantitative receptor density was measured through all cortical layers at each developmental stage. Pharmacological identification of I-125-pPPY binding sites was made by competition experiments using pNPY or [Leu(31), Pro(34)]pNPY and pNPY(13-36) as selective competitors for Y1 and Y2 receptors, respectively. NPY receptors were first detected in the cerebral cortex at low densities at E19 in a thin layer of tissue corresponding to the inner half of the intermediate zone (IZ) and the upper ventricular zone (VZ). The neuroepithelium didnot contain binding sites. High densities of sites were observed by E21 onward to P10 in the deep cortical layers corresponding to the IZ and layers V-VL A decreasing gradient of receptor density was observed from layer VI to the marginal zone (layer I). The distribution of NPY receptors does not match with the perikarya of transient NPY-immunoreactive neurons located in the cortical plate but does coincide with their axonal extension. The receptor density decreased abruptly between P10 and P12 in deep layers, whereas a moderate expression of binding sites is detected from P10 to P12 in layers I-III. By P14, the binding level was the lowest observed in the postnatalperiod. From P21 onward, receptors were observed in superficial layers I-III, and their density rose by two- to threefold up to adulthood. Competition studies indicated that the NPY receptors located in the deep cortical layers of the E21 or P1 rat cortex exhibit Y2 receptor type characteristics. The binding sites detected in the superficial layers from P10 to P12 rats also show Y2 receptors characteristics, unlike the NPY receptors in layers II-III of the adult, which behave like Y1 receptors. These data show that different NPY receptor types are successively expressed in specific layers during late gestation and early postnatal life in the rat frontoparietal cortex. (C) 2002 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/20 alle ore 01:22:01