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Titolo:
Detection and localization of Cripto-1 binding in mouse mammary epithelialcells and in the mouse mammary gland using an immunoglobulin-Cripto-1 fusion protein
Autore:
Bianco, C; Normanno, N; De Luca, A; Maiello, MR; Wechselberger, C; Sun, Y; Khan, N; Adkins, H; Sanicola, M; Vonderhaar, B; Cohen, B; Seno, M; Salomon, D;
Indirizzi:
NCI, TGFS, BRI, NIH, Bethesda, MD 20892 USA NCI Bethesda MD USA 20892NCI, TGFS, BRI, NIH, Bethesda, MD 20892 USA ITN Fdn Pascale, Oncol Sperimentale D, Naples, Italy ITN Fdn Pascale Naples Italy scale, Oncol Sperimentale D, Naples, Italy NCI, Mol Endocrinol Sect, Basic Res Lab, NIH, Bethesda, MD 20892 USA NCI Bethesda MD USA 20892 ect, Basic Res Lab, NIH, Bethesda, MD 20892 USA Pfizer Cent Res Div, Groton, CT USA Pfizer Cent Res Div Groton CT USAPfizer Cent Res Div, Groton, CT USA Okayama Univ, Fac Engn, Dept Biosci & Biotechnol, Okayama, Japan Okayama Univ Okayama Japan gn, Dept Biosci & Biotechnol, Okayama, Japan Biogen Inc, Cambridge, MA 02142 USA Biogen Inc Cambridge MA USA 02142Biogen Inc, Cambridge, MA 02142 USA
Titolo Testata:
JOURNAL OF CELLULAR PHYSIOLOGY
fascicolo: 1, volume: 190, anno: 2002,
pagine: 74 - 82
SICI:
0021-9541(200201)190:1<74:DALOCB>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
TYROSINE PHOSPHORYLATION; VERTEBRATE DEVELOPMENT; TRANSGENIC MICE; BREAST-CANCER; GENE FAMILY; GROWTH; EXPRESSION; AMPHIREGULIN; RECEPTOR; GASTRULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Salomon, D NCI, TGFS, BRI, NIH, Bldg 10,Rm 5B39,9000 Rockville Pike, Bethesda, MD 20892 USA NCI Bldg 10,Rm 5B39,9000 Rockville Pike Bethesda MD USA 20892 A
Citazione:
C. Bianco et al., "Detection and localization of Cripto-1 binding in mouse mammary epithelialcells and in the mouse mammary gland using an immunoglobulin-Cripto-1 fusion protein", J CELL PHYS, 190(1), 2002, pp. 74-82

Abstract

Human Cripto-1 (CR-1), a member of the epidermal growth factor-CFC (EGF-CFC) family of peptides, is expressed in the developing mouse mammary gland and can modulate mammary epithelial cell migration, branching morphogenesis and milk protein expression in vitro. In order to screen for a CR-1 receptor and to identify potential CR-1 target tissues, we constructed a fusion protein comprising the EGF-like domain of CR-1 and the Fc domain of a human IgG1. The recombinant CR-1 fusion protein (CR-1-Fc) was biologically active as it was able to activate the ras/raf/mitogen activated protein kinase (MAPK) pathway and to inhibit transcription of the milk protein P-casein in NMuMG and HC-11 mouse mammary epithelial cells. By using immunocytochemistry and by an in situ enzyme-linked immunosorbent assay (ELISA), CR-1-Fc was found to specifically bind to NMuMG and HC-11 cells. Finally, immunohistochemical analysis using CR-1-Fc showed a specific localization of CR-1 binding to tissue sections from mouse mammary gland. In particular, more than 60% of the epithelial cells were intensely stained with the CR-1-Fc fusion protein in the lactating mouse mammary gland, whereas approximately 25% of the mammary epithelial cells were stained in the gland from pregnant mouse. Since expression of mouse cripto-1 (Cr-1) in the pregnant and lactating mouse mammary gland as well as its presence in milk has been previously demonstrated, these data strongly suggest that an autocrine pathway involving Cr-1 and its putative receptor is operating in the mouse mammary gland during pregnancy and lactation. Published 2002 Wiley-Liss, Inc.(dagger)

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 19:19:12