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Titolo:
Basic fibroblast growth factor protects cardiac myocytes from iNOS-mediated apoptosis
Autore:
Iwai-Kanai, E; Hasegawa, K; Fujita, M; Araki, M; Yanazume, T; Adachi, S; Sasayama, S;
Indirizzi:
Kyoto Univ, Grad Sch Med, Dept Cardiovasc Med, Sakyo Ku, Kyoto 6068507, Japan Kyoto Univ Kyoto Japan 6068507 ovasc Med, Sakyo Ku, Kyoto 6068507, Japan Kyoto Univ, Coll Med Technol, Kyoto 6068507, Japan Kyoto Univ Kyoto Japan 6068507 v, Coll Med Technol, Kyoto 6068507, Japan Kyoto Univ, Grad Sch Med, Dept Pediat, Kyoto 6068507, Japan Kyoto Univ Kyoto Japan 6068507 ch Med, Dept Pediat, Kyoto 6068507, Japan
Titolo Testata:
JOURNAL OF CELLULAR PHYSIOLOGY
fascicolo: 1, volume: 190, anno: 2002,
pagine: 54 - 62
SICI:
0021-9541(200201)190:1<54:BFGFPC>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE SYNTHASE; HUMAN HEART; INFARCT SIZE; EXPRESSION; MYOCARDIUM; CARDIOMYOCYTES; INDUCTION; FAILURE; GENE; REPERFUSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Hasegawa, K Kyoto Univ, Grad Sch Med, Dept Cardiovasc Med, Sakyo Ku, 54 Kawara Cho, Kyoto 6068507, Japan Kyoto Univ 54 Kawara Cho Kyoto Japan 6068507 o 6068507, Japan
Citazione:
E. Iwai-Kanai et al., "Basic fibroblast growth factor protects cardiac myocytes from iNOS-mediated apoptosis", J CELL PHYS, 190(1), 2002, pp. 54-62

Abstract

Basic fibroblast growth factor (bFGF) is an important angiogenic factor produced by hearts subjected to ischemia. However, the direct effects of bFGFon myocardial cells are unknown. Primary cultured cardiac myocytes from neonatal rats were stimulated with lipopolysaccharide (LPS), a potent inducerof inducible nitric oxide synthase (iNOS), in the presence or the absence of bFGF. LPS induced the expression of iNOS in cardiac myocytes, demonstrated at both mRNA and protein levels. We showed that LPS activated the apoptotic pathway, evidenced by TUNEL staining, DNA ladder formation, and morphologic features. LPS-induced apoptosis was blocked by the administration of L-NAME, an inhibitor of NOS. This indicates that LPS induces apoptosis via an iNOS-dependent pathway. Administration of bFGF completely inhibited myocardial cell apoptosis induced by hydrogen peroxide or acidic medium as well as LPS. To determine signaling pathways for this inhibitory effect, We utilized PD098059, an MEK-1-specific inhibitor. PD098059 blocked bFGF-induced activation of ERK (extracellularly responsive kinase)-1/2 and neutralized the apoptotic inhibitory effect of bFGF. These findings demonstrate that LPS induces myocardial cell apoptosis in an iNOS-dependent manner. The results also suggest that bFGF is a protective factor against myocardial cell apoptosis and that this protection requires the MEK-1-ERK pathway. (C) 2002 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 09:03:35