Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Direct targeting of cis-Golgi matrix proteins to the Golgi apparatus
Autore:
Yoshimura, S; Nakamura, N; Barr, FA; Misumi, Y; Ikehara, Y; Ohno, H; Sakaguchi, M; Mihara, K;
Indirizzi:
Kanazawa Univ, Canc Res Inst, Kanazawa, Ishikawa 9200934, Japan Kanazawa Univ Kanazawa Ishikawa Japan 9200934 wa, Ishikawa 9200934, Japan Kyushu Univ, Grad Sch Med Sci, Dept Mol Biol, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 Dept Mol Biol, Fukuoka 8128582, Japan Max Planck Inst Biochem, Dept Cell Biol, D-82152 Martinsried, Germany Max Planck Inst Biochem Martinsried Germany D-82152 Martinsried, Germany Fukuoka Univ, Sch Med, Dept Biochem, Fukuoka 8140180, Japan Fukuoka Univ Fukuoka Japan 8140180 Dept Biochem, Fukuoka 8140180, Japan
Titolo Testata:
JOURNAL OF CELL SCIENCE
fascicolo: 22, volume: 114, anno: 2001,
pagine: 4105 - 4115
SICI:
0021-9533(200111)114:22<4105:DTOCMP>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOPLASMIC-RETICULUM; RETROGRADE TRANSPORT; PLASMA-MEMBRANE; COPI VESICLES; HELA-CELLS; GM130; P115; BINDING; MITOSIS; IDENTIFICATION;
Keywords:
Golgi; membrane binding; vesicular transport; subcellular fractionation; microinjection; mutant Sar1p;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Nakamura, N Kanazawa Univ, Canc Res Inst, Kanazawa, Ishikawa 9200934, Japan Kanazawa Univ Kanazawa Ishikawa Japan 9200934 9200934, Japan
Citazione:
S. Yoshimura et al., "Direct targeting of cis-Golgi matrix proteins to the Golgi apparatus", J CELL SCI, 114(22), 2001, pp. 4105-4115

Abstract

The targeting route of newly synthesized GM130 and GRASP65 to the Golgi apparatus was investigated by three different approaches. First, localizationof pulse labeled GM130 and GRASP65 in normal rat kidney (NRK) cells was traced by subcellular fractionation followed by immunoprecipitation. Immediately after the pulse labeling, GM130 and GRASP65 were found in the Golgi butnot in the endoplasmic reticulum (ER) membrane fractions, whereas a control Golgi membrane protein was still found in the ER membrane fractions. Second, epitope tagged GM130 and GRASP65 were expressed in NRK cells by plasmidmicroinjection into the nuclei and their localization was analyzed by immunofluorescence. When ER to Golgi transport was inhibited by prior microinjection of a GTP-restricted mutant of Sar1 protein into the cytosol, the expressed GM130 and GRASP65 showed clear Golgi localization. Last, binding of GM130 and GRASP65 to the membranes was analyzed in vitro. In vitro synthesized GM130 and GRASP65 specifically bound to purified Golgi membranes but notto microsomal membranes. The bound GM130 and GRASP65 were found to form a complex with pre-existing counterparts on the Golgi membrane. These resultsstrongly suggested that GM130 and GRASP65 are directly targeted to the Golgi membrane without initial assembly on the ER and subsequent vesicular transport to the Golgi apparatus.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/21 alle ore 18:16:58