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Titolo:
Vesicle-associated membrane protein of Arabidopsis suppresses Bax-induced apoptosis in yeast downstream of oxidative burst
Autore:
Levine, A; Belenghi, B; Damari-Weisler, H; Granot, D;
Indirizzi:
Hebrew Univ Jerusalem, Dept Plant Sci, IL-91904 Jerusalem, Israel Hebrew Univ Jerusalem Jerusalem Israel IL-91904 -91904 Jerusalem, Israel Agr Res Org, Volcani Ctr, Inst Field & Garden Crops, IL-50250 Bet Dagan, Israel Agr Res Org Bet Dagan Israel IL-50250 Crops, IL-50250 Bet Dagan, Israel
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 49, volume: 276, anno: 2001,
pagine: 46284 - 46289
SICI:
0021-9258(200112)276:49<46284:VMPOAS>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; DISEASE RESISTANCE RESPONSE; VESICULAR TRANSPORT; SUSPENSION-CULTURES; MEDIATED APOPTOSIS; THALIANA CDNAS; PLANTS; FUSION; TRANSCRIPTION; INVOLVEMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Levine, A Hebrew Univ Jerusalem, Dept Plant Sci, IL-91904 Jerusalem, Israel Hebrew Univ Jerusalem Jerusalem Israel IL-91904 usalem, Israel
Citazione:
A. Levine et al., "Vesicle-associated membrane protein of Arabidopsis suppresses Bax-induced apoptosis in yeast downstream of oxidative burst", J BIOL CHEM, 276(49), 2001, pp. 46284-46289

Abstract

Programmed cell death (PCD) in many systems is controlled by relative amounts of the apoptosis-regulating proteins Bax and Bcl-2 through homo- or heterodimerization. Here we show that Bax-induced PCD of yeast was suppressed by transformation with a vesicle-associated membrane protein from Arabidopsis (AtVAMP), which was isolated by screening a cDNA expression library against sugar-induced cell death in yeast. AtVAMP expression blocked Bax-induced PCD downstream of oxidative burst. AtVAMP also prevented H2O2-induced apoptosis in yeast and in Arabidopsis cells. Reduced oxidation of lipids and plasma membrane proteins was detected in the AtVAMP-transformed yeast, suggesting improved membrane repair. Inhibition of intracellular vesicle trafficking by brefeldin A induced apoptosis from a sublethal concentration of H2O2. No protection occurred by overexpression of the yeast homolog SCN2. However, efficient suppression of yeast PCD occurred by expression of a chimeric gene, composed of the conserved domains from yeast, fused to the variableN-terminal domain from Arabidopsis, resulting in exchange of the proline-rich N-terminal domain of SCN2 with a proline-poor Arabidopsis sequence. Ourresults suggest that intracellular vesicle traffic can regulate execution of apoptosis by affecting the rate of membrane recycling and that the proline-rich N-terminal domain of VAMP inhibited this process.

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Documento generato il 15/01/21 alle ore 23:07:17