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Titolo:
Cell-derived apolipoprotein E (ApoE) particles inhibit vascular cell adhesion molecule-1 (VCAM-1) expression in human endothelial cells
Autore:
Stannard, AK; Riddell, DR; Sacre, SM; Tagalakis, AD; Langer, C; von Eckardstein, A; Cullen, P; Athanasopoulos, T; Dickson, G; Owen, JS;
Indirizzi:
Univ Coll London, Dept Med, Royal Free & Univ Coll, Sch Med, London NW3 2PF, England Univ Coll London London England NW3 2PF Sch Med, London NW3 2PF, England Univ Munster, Inst Klin Chem & Lab Med, D-48149 Munster, Germany Univ Munster Munster Germany D-48149 & Lab Med, D-48149 Munster, Germany Univ Munster, Inst Arterioskleroseforsch, D-48149 Munster, Germany Univ Munster Munster Germany D-48149 oseforsch, D-48149 Munster, Germany Univ London Royal Holloway & Bedford New Coll, Sch Biol Sci, Egham TW20 0EX, Surrey, England Univ London Royal Holloway & Bedford New Coll Egham Surrey England TW20 0EX
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 49, volume: 276, anno: 2001,
pagine: 46011 - 46016
SICI:
0021-9258(200112)276:49<46011:CAE(PI>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-BLOOD-PLATELETS; NITRIC-OXIDE; LIPOPROTEIN METABOLISM; GENE-EXPRESSION; DEFICIENT MICE; VLDL RECEPTOR; ATHEROSCLEROSIS; MACROPHAGES; CHOLESTEROL; AGGREGATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Owen, JS Univ Coll London, Dept Med, Royal Free & Univ Coll, Sch Med, Rowland Hill St, London NW3 2PF, England Univ Coll London Rowland Hill St London England NW3 2PF England
Citazione:
A.K. Stannard et al., "Cell-derived apolipoprotein E (ApoE) particles inhibit vascular cell adhesion molecule-1 (VCAM-1) expression in human endothelial cells", J BIOL CHEM, 276(49), 2001, pp. 46011-46016

Abstract

Sub-endothelial infiltration of monocytes occurs early in atherogenesis and is facilitated by cell adhesion molecules that are up-regulated on activated endothelium. Apolipoprotein E (apoE) helps protect against atherosclerosis, in part, because apoE particles secreted by macrophages have local beneficial effects at lesion sites. Here, we hypothesize that such protection includes antiinflammatory actions and investigate whether cell-derived apoEcan inhibit tumor necrosis factor-a-mediated up-regulation of vascular cell adhesion molecule-1 (VCAM-1) in human umbilical vein endothelial cells (HuVECs). Two models were used to mimic endothelial exposure to macrophage-derived apoE. In the first, HUVECs were transiently transfected to secrete apoE; VCAM-1 induction inversely correlated with secretion of apoE into the media (r = -0.76, p < 0.001). In the second, incubation of HUVECs with mediafrom recombinant Chinese hamster ovary (CHO) cells expressing apoE (CHOapoE) also reduced VCAM-1 in a dose-dependent manner (r = -0.70, p < 0.001). Characterization of CHOapoE cell-derived apoE revealed several similarities to apoE particles secreted by human blood monocyte-derived macrophages. Thesuppression of endothelial activation by apoE most likely occurs via stimulation of endothelial nitric oxide synthase; apoE increased levels of intracellular nitric oxide and its surrogate marker, cyclic guanosine monophosphate, while the nitric oxide synthase inhibitor, ethyl-isothiourea, blocked its effect. We propose that apoE secreted locally at lesion sites by macrophages may be anti-inflammatory by stimulating endothelium to release NO andsuppress VCAM-1 expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 10:05:49