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Titolo:
SRY interacts with and negatively regulates androgen receptor transcriptional activity.
Autore:
Yuan, X; Lu, ML; Li, T; Balk, SP;
Indirizzi:
Beth Israel Deaconess Med Ctr, Div Hematol Oncol, Dept Med, Canc Biol Program, Boston, MA 02215 USA Beth Israel Deaconess Med Ctr Boston MA USA 02215 m, Boston, MA 02215 USA Harvard Univ, Sch Med, Boston, MA 02215 USA Harvard Univ Boston MA USA 02215 vard Univ, Sch Med, Boston, MA 02215 USA Brigham & Womens Hosp, Dept Urol, Boston, MA 02215 USA Brigham & Womens Hosp Boston MA USA 02215 Dept Urol, Boston, MA 02215 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 49, volume: 276, anno: 2001,
pagine: 46647 - 46654
SICI:
0021-9258(200112)276:49<46647:SIWANR>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADRENAL HYPOPLASIA CONGENITA; PROSTATE-SPECIFIC ANTIGEN; LIGAND-BINDING DOMAIN; INHIBITING SUBSTANCE GENE; ESTROGEN RESPONSE ELEMENT; TESTIS-DETERMINING FACTOR; AUTOSOMAL SEX REVERSAL; AMINO-TERMINAL DOMAIN; NUCLEAR-RECEPTOR; DNA-BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
78
Recensione:
Indirizzi per estratti:
Indirizzo: Yuan, X Beth Israel Deaconess Med Ctr, Div Hematol Oncol, Dept Med, Canc Biol Program, 330 Brookline Ave, Boston, MA 02215 USA Beth Israel Deaconess Med Ctr 330 Brookline Ave Boston MA USA 02215
Citazione:
X. Yuan et al., "SRY interacts with and negatively regulates androgen receptor transcriptional activity.", J BIOL CHEM, 276(49), 2001, pp. 46647-46654

Abstract

This study investigated interactions between SRY, the Y chromosome encodedmale sex determining factor, and the androgen receptor (AR). Coexpression of AR and SRY caused marked repression of AR transcriptional activity on a series of androgen-responsive reporter genes. Mammalian one- and two-hybridexperiments demonstrated an AR-SRY interaction mediated by the AR DNA binding domain. Precipitations with glutathione S-transferase fusion proteins indicated that AR-SRY interactions were direct and mediated by the AR DNA binding domain and the SRY high mobility group box DNA binding domain. Transient expression of SRY in LNCaP prostate cancer cells repressed expression of an androgen-dependent prostate-specific antigen (PSA) reporter gene and stable SRY expression repressed the endogenous PSA gene. SRY protein expression was increased by proteosome inhibitors and by the androgen-liganded AR in transient and stable transfectants. AR transcriptional activity was alsorepressed by DAX1, and the effects of SRY and DAX1 on the AR were additive. These findings indicate that interactions between the AR, SRY, and DAX1 contribute to normal male development and function and suggest a general role for protein-protein interactions between high mobility group box proteinsand steroid hormone receptors in regulating tissue-specific gene expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 08:30:16