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Titolo:
Requirements for allergen-induced airway inflammation and hyperreactivity in CD4-deficient and CD4-sufficient HLA-DQ transgenic mice
Autore:
Chapoval, SP; Marietta, EV; Smart, MK; David, CS;
Indirizzi:
Mayo Clin, Dept Immunol, Rochester, MN 55905 USA Mayo Clin Rochester MN USA 55905 n, Dept Immunol, Rochester, MN 55905 USA
Titolo Testata:
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
fascicolo: 5, volume: 108, anno: 2001,
pagine: 764 - 771
SICI:
0091-6749(200111)108:5<764:RFAAIA>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLLAGEN-INDUCED ARTHRITIS; SHORT RAGWEED ALLERGEN; MAST-CELLS; ASTHMA MODEL; EOSINOPHIL INFILTRATION; HISTAMINE-RELEASE; HUMAN CD4; T-CELLS; HYPERRESPONSIVENESS; EXPRESSION;
Keywords:
MHC; HLA; human CD4; transgenic/knockout; short ragweed allergen; in vivo animal models; asthma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: David, CS Mayo Clin, Dept Immunol, Rochester, MN 55905 USA Mayo Clin Rochester MN USA 55905 munol, Rochester, MN 55905 USA
Citazione:
S.P. Chapoval et al., "Requirements for allergen-induced airway inflammation and hyperreactivity in CD4-deficient and CD4-sufficient HLA-DQ transgenic mice", J ALLERG CL, 108(5), 2001, pp. 764-771

Abstract

Background: Airway inflammation is central to the pathogenesis of allergicasthma, and molecules that mediate this process obviously represent targets for therapy. Objective: To study the role of CD4+ T cells and/or HLA-DQ molecules in allergic asthma, we have generated and characterized models of short ragweed allergen (SRW)-induced inflammation using transgenic mice with HLA-DQ (DQ6 or DQ8), human CD4 (hCD4), or both on a genetic background that lacks mouseMHC II and CD4 (A beta (0)/mCD4(0)). Methods: Mice were actively sensitized and later challenged intranasally with SRW allergenic extract. Bronchoalveolar lavage fluid composition, airway inflammation and hyperresponsiveness, blood eosinophil levels, and cell proliferation were examined. Results: In response to SRW treatment, both DQ6 and DQ8 transgenic mice expressing hCD4 developed pulmonary eosinophilia and associated lung tissue damage with increase in eosinophil peroxidase and T(H)2 cytokines in bronchoalveolar lavage fluid, strong airway hyperreactivity, and persistent blood eosinophilia. The response was independent of mast cells/histamine pathway and was mediated by DQ-restricted hCD4+ T cells. Interestingly, lungs of CD4-deficient DQ6 transgenic mice showed an eosinophilic inflammation withoutlocal increase in cytokines and eosinophil peroxidase. The allergic reaction was absent in double-knockout mice and mice expressing either DQ8 or hCD4 alone. Conclusions: DQ6 molecules are critical to SRW-induced allergy and can operate in the presence or absence of CD4. However, both DQ antigens and CD4 molecules are critical for full manifestation of allergen-induced asthma in transgenic mice.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 08:37:13