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Titolo:
Improved detection of melanoma antigen-specific T cells expressing low or high levels of CD8 by HLA-A2 tetramers presenting a Melan-A/MART-1 peptide analogue
Autore:
Maeurer, MJ; Necker, A; Salter, RD; Castelli, C; Hohn, H; Karbach, J; Freitag, K; Neukirch, C; Knuth, A; Jager, E;
Indirizzi:
Univ Mainz, Dept Med Microbiol, D-55101 Mainz, Germany Univ Mainz Mainz Germany D-55101 t Med Microbiol, D-55101 Mainz, Germany Beckmann Coulter, Immun Operat, Marseille, France Beckmann Coulter Marseille France lter, Immun Operat, Marseille, France Univ Pittsburgh, Sch Med, Dept Pathol, Pittsburgh, PA USA Univ PittsburghPittsburgh PA USA h Med, Dept Pathol, Pittsburgh, PA USA Ist Tumori Milano, Milan, Italy Ist Tumori Milano Milan ItalyIst Tumori Milano, Milan, Italy Med Klin 2, Frankfurt, Germany Med Klin 2 Frankfurt GermanyMed Klin 2, Frankfurt, Germany
Titolo Testata:
INTERNATIONAL JOURNAL OF CANCER
fascicolo: 1, volume: 97, anno: 2002,
pagine: 64 - 71
SICI:
0020-7136(20020101)97:1<64:IDOMAT>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOTOXIC LYMPHOCYTES-T; ALPHA-3 DOMAIN; RECEPTOR-BINDING; CLASS-I; IDENTIFICATION; REPERTOIRE; ACTIVATION; EXPANSIONS; MULTIMERS; COMPLEXES;
Keywords:
major histocompatibility complex class I; HLA-A2; T-cell receptor; CD8; Melan-A/MART-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Maeurer, MJ Univ Mainz, Dept Med Microbiol, Hochhaus Augustuspl, D-55101 Mainz, Germany Univ Mainz Hochhaus Augustuspl Mainz Germany D-55101 Germany
Citazione:
M.J. Maeurer et al., "Improved detection of melanoma antigen-specific T cells expressing low or high levels of CD8 by HLA-A2 tetramers presenting a Melan-A/MART-1 peptide analogue", INT J CANC, 97(1), 2002, pp. 64-71

Abstract

MHC class I tetramers containing peptide epitopes are sensitive tools for detecting antigen-specific CD8(+) T-cell responses. We demonstrate here that binding of HLA-A2 tetramers to CD8(+) T cells specific for the melanoma-associated antigen Melan-A/MART-I can be fine-tuned by altering either the bound peptide epitope or residues in the alpha3 domain of HLA-A2, which is important for CD8 binding. Antigen-specific T cells expressing high levels of CD8 could be detected using HLA-A2 tetramers containing the peptide AAGIGILTV, an epitope which is naturally processed and presented from Melan-A/MART-I. In contrast, low CD8-expressing, antigen-specific T cells could be detected efficiently only by using a mutated HLA-A2 tetramer with an altered CD8 binding site or, less efficiently, using the wild-type HLA-A2 tetramer loaded with the peptide analogue ELAGIGILTV, which is superior in stimulating antigen-specific T-cell responses. Our results suggest ways to optimize the identification and expansion of antigen-specific T cells with differentrequirements for the costimulatory CD8 molecule in facilitating T-cell receptor binding to peptide variants. (C) 2002 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 03:55:16