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Titolo:
Bipartite regulation of different components of the MHC class I antigen-processing machinery during dendritic cell maturation
Autore:
Li, J; Schuler-Thurner, B; Schuler, G; Huber, C; Seliger, B;
Indirizzi:
Univ Mainz, Dept Internal Med 3, D-55131 Mainz, Germany Univ Mainz MainzGermany D-55131 Internal Med 3, D-55131 Mainz, Germany Univ Erlangen Nurnberg, Dept Dermatol, D-91052 Erlangen, Germany Univ Erlangen Nurnberg Erlangen Germany D-91052 -91052 Erlangen, Germany
Titolo Testata:
INTERNATIONAL IMMUNOLOGY
fascicolo: 12, volume: 13, anno: 2001,
pagine: 1515 - 1523
SICI:
0953-8178(200112)13:12<1515:BRODCO>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXOGENOUS PROTEIN ANTIGENS; EPIDERMAL LANGERHANS CELLS; T-CELLS; BONE-MARROW; INFLAMMATORY STIMULI; HUMAN BLOOD; MATURE; GENERATION; EXPRESSION; CYTOKINES;
Keywords:
antigen processing; dendritic cells; immune response; MHC;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Seliger, B Univ Mainz, Dept Internal Med 3, D-55131 Mainz, Germany Univ Mainz Mainz Germany D-55131 ed 3, D-55131 Mainz, Germany
Citazione:
J. Li et al., "Bipartite regulation of different components of the MHC class I antigen-processing machinery during dendritic cell maturation", INT IMMUNOL, 13(12), 2001, pp. 1515-1523

Abstract

Dendritic cells (DC) are professional antigen-presenting cells (APC) whichproceed from immature to a mature stage during their final differentiation. Immature DC are highly effective in terms of antigen uptake and processing, whereas mature DC become potent immunostimulatory cells. Until now, the expression profiles of the major components of the MHC class I antigen-processing machinery (APM) during DC development have not been well characterized. In this study, the mRNA and protein expression levels of the IFN-gamma inducible proteasome subunits, of the proteasome activators PA28, and of key components required for peptide transport and MHC class I-peptide complexassembly have been evaluated in immature and mature stages of human monocyte-derived DC using semiquantitative RT-PCR and Western blot analyses. The IFN-gamma -responsive immunoproteasome subunits LMP2, LMP7 and MECL1 are up-regulated in immature DC, whereas the other components of the MHC class I presentation machinery, such as PA28, TAP, tapasin, and HLA heavy and lightchains, were found to be more abundant in mature DC. These findings support the hypothesis that immature DC produced by the differentiation of monocytes in response to IL-4 and granulocyte macrophage colony stimulating factor first increase their capacity to capture antigens and process them into peptides, thereby switching from housekeeping to immunoproteasomes, while mature DC rather up-regulate the components required for peptide translocation and MHC class I-peptide complex formation, and thus specialize in antigenpresentation. Our results establish that MHC class I, like MHC class II surface expression, is markedly regulated during DC development and maturation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 00:19:29