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Titolo:
Infection of murine keratinocytes with herpes simplex virus type 1 inducesthe expression of interleukin-10, but not interleukin-1 alpha or tumour necrosis factor-alpha
Autore:
Zak-Prelich, M; Halliday, KE; Walker, C; Yates, CM; Norval, M; McKenzie, RC;
Indirizzi:
Med Univ Lodz, Dept Dermatol, Lodz, Poland Med Univ Lodz Lodz PolandMed Univ Lodz, Dept Dermatol, Lodz, Poland Univ Edinburgh, Sch Med, Dept Dermatol, Edinburgh, Midlothian, Scotland Univ Edinburgh Edinburgh Midlothian Scotland burgh, Midlothian, Scotland Univ Edinburgh, Sch Med, Dept Med Microbiol, Edinburgh EH8 9AG, Midlothian, Scotland Univ Edinburgh Edinburgh Midlothian Scotland EH8 9AG Midlothian, Scotland
Titolo Testata:
IMMUNOLOGY
fascicolo: 4, volume: 104, anno: 2001,
pagine: 468 - 475
SICI:
0019-2805(200112)104:4<468:IOMKWH>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
DELAYED-TYPE HYPERSENSITIVITY; EARLY GENE-EXPRESSION; IFN-GAMMA; IL-10; CELL; LESIONS; MUTANT; MODULATION; CLEARANCE; PROTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: McKenzie, RC Royal Infirm, Dept Dermatol, Lauriston Pl, Edinburgh EH3 9YW,Midlothian, Scotland Royal Infirm Lauriston Pl Edinburgh Midlothian Scotland EH3 9YW
Citazione:
M. Zak-Prelich et al., "Infection of murine keratinocytes with herpes simplex virus type 1 inducesthe expression of interleukin-10, but not interleukin-1 alpha or tumour necrosis factor-alpha", IMMUNOLOGY, 104(4), 2001, pp. 468-475

Abstract

Herpes simplex virus (HSV) is known to possess several mechanisms whereby,it can evade the normal host immune defences. In this study the expressionof the immunosuppressive cytokine, interleukin (IL)-10, was monitored following infection of a murine keratinocyte cell line(PAM-212) and compared with the expression of two proinflammatory cytokines: IL-1 alpha and tumour necrosis factor (TNF)-alpha. The PAM-212 cells were infected at a multiplicity of 0.5 with a clinical isolate of HSV type 1, and the mRNA of the three cytokines was assessed by semiquantitative reverse transcription-polymerasechain reaction (RT-PCR) over the following 24 hr. By 12 hr postinfection the amount of IL-10 mRNA had increased significantly to five-fold greater than that found in uninfected cells (P<0.01), and this elevated level was maintained until at least 24 hr postinfection. In contrast, IL-1<alpha> and TNF-alpha mRNAs were not significantly up-regulated by the HSV infection. Immunostaining with an IL-10 monoclonal antibody (mAb) revealed that cytoplasmic IL-10 protein had increased by 6-12 hr postinfection. This quantity was further increased at 24 hr postinfection, when the viral cytopathic effect was apparent. Viral replication was necessary, but not sufficient on its own, for IL-10 induction. Experiments with HSV mutants lacking functional transactivating factors suggested that the viral transactivating proteins ICP-0 and VP-16 may be necessary for HSV-induced IL-10 expression. Thus, the up-regulation in the expression of IL-10 mRNA and protein induced by HSV early in the infection of keratinocytes represents a specific response and may be part of the viral strategy to avoid local immune defence mechanisms in the skin.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 14:14:46