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Titolo:
Role of calcium influx in cytotoxic T lymphocyte lytic granule exocytosis during target cell killing
Autore:
Lyubchenko, TA; Wurth, GA; Zweifach, A;
Indirizzi:
Univ Colorado, Hlth Sci Ctr, Dept Physiol & Biophys, Denver, CO 80262 USA Univ Colorado Denver CO USA 80262 Physiol & Biophys, Denver, CO 80262 USA Univ Colorado, Hlth Sci Ctr, Dept Immunol, Denver, CO 80262 USA Univ Colorado Denver CO USA 80262 Ctr, Dept Immunol, Denver, CO 80262 USA
Titolo Testata:
IMMUNITY
fascicolo: 5, volume: 15, anno: 2001,
pagine: 847 - 859
SICI:
1074-7613(200111)15:5<847:ROCIIC>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
NATURAL-KILLER-CELLS; MICROTUBULE-ORGANIZING CENTER; GOLGI-APPARATUS; MAST-CELLS; CA2+; ACTIVATION; BINDING; ANTIGEN; RELEASE; DEGRANULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Zweifach, A Univ Colorado, Hlth Sci Ctr, Dept Physiol & Biophys, 4200 E 9th Ave, Denver, CO 80262 USA Univ Colorado 4200 E 9th Ave Denver CO USA 80262 CO 80262 USA
Citazione:
T.A. Lyubchenko et al., "Role of calcium influx in cytotoxic T lymphocyte lytic granule exocytosis during target cell killing", IMMUNITY, 15(5), 2001, pp. 847-859

Abstract

One mechanism cytotoxic T lymphocytes use to kill targets is exocytosis ofcytotoxic agents from lytic granules, a process that requires Ca2+ influx. We investigated the role of Ca2+ influx in granule exocytosis using TALL-104 human leukemic cytotoxic T cells triggered via a bispecific antibody containing an anti-CD3 F(ab') to kill Raji B lymphoma cells. Using a novel fluorescence method, we detected target-directed release of similar to 15% of lytic granules during killing. Consistent with previous work, we observed sustained CTL Ca2+ gradients during killing, but gradients reflect the behavior of Fura-2 in granules. Rapid imaging experiments suggest that Ca2+ channels are not polarized during killing, indicating that Ca2+ influx does notdirect granule reorientation. Furthermore, we find that Ca2+ acts via a high-affinity interaction to promote granule exocytosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 11:05:16