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Titolo:
Cre-mediated transgene activation in the developing and adult mouse brain
Autore:
Cinato, E; Mirotsou, M; Sablitzky, F;
Indirizzi:
Univ Nottingham, Queens Med Ctr, Genet Inst, Nottingham NG7 2UH, England Univ Nottingham Nottingham England NG7 2UH , Nottingham NG7 2UH, England Univ Coll London, Windeyer Inst Med Sci, Dept Med, London WC1E 6BT, England Univ Coll London London England WC1E 6BT t Med, London WC1E 6BT, England
Titolo Testata:
GENESIS
fascicolo: 3, volume: 31, anno: 2001,
pagine: 118 - 125
SICI:
1526-954X(200111)31:3<118:CTAITD>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
SITE-SPECIFIC RECOMBINATION; EMBRYONIC STEM-CELLS; DNA RECOMBINATION; MICE; EXPRESSION; BACTERIOPHAGE-P1; PROMOTER; DELETION; ENOLASE; MEMORY;
Keywords:
rat enolase promoter; site-specific recombination; loxP sites; neurogenesis; dormant lacZ indicator mice;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Sablitzky, F Univ Nottingham, Queens Med Ctr, Genet Inst, Nottingham NG7 2UH, England Univ Nottingham Nottingham England NG7 2UH NG7 2UH, England
Citazione:
E. Cinato et al., "Cre-mediated transgene activation in the developing and adult mouse brain", GENESIS, 31(3), 2001, pp. 118-125

Abstract

The neuron-specific rat enolase (NSE) promoter was employed to establish transgenic mice expressing Cre recombinase in the central nervous system. Founders were crossed with dormant lacZ indicator mice and specificity as well as efficiency of Cre-mediated transgene activation was determined by PCR and/or X-gal staining. Whereas most transgenic lines exhibited Cre activityin early development resulting in widespread Cre activity, one line (NSE-Cre26) expressed high levels of Cre in the developing and adult brain. With the exception of kidney, which showed occasionally low level of Cre activity, Cre recombination in double transgenics was restricted to the nervous system. Wholemount X-gal staining of 9.5 dpc embryos indicated Cre-mediated lacZ expression in forebrain, hindbrain, and along the midbrain flexure. A similar expression pattern was observed during later stages of embryogenesis(11.5-13.5 dpc). In adult mice, Cre recombinase was expressed in cerebral cortex and cerebellum and high levels of Cre-mediated lacZ expression were observed in hippocampus, cortex, and septum. The NSE-Cre26 transgenic mouseline thus provides a useful tool to specifically overexpress and/or inactivate genes in the developing and adult brain. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 15:59:10