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Titolo:
Isolation and characterization of chicken TaxREB107, a putative DNA binding protein abundantly expressed in muscle
Autore:
Thomson, SR; Johnson, SE;
Indirizzi:
Penn State Univ, Dept Poultry Sci, University Pk, PA 16802 USA Penn State Univ University Pk PA USA 16802 i, University Pk, PA 16802 USA
Titolo Testata:
GENE
fascicolo: 1-2, volume: 278, anno: 2001,
pagine: 81 - 88
SICI:
0378-1119(20011031)278:1-2<81:IACOCT>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
TAX-RESPONSIVE ENHANCER; LONG TERMINAL REPEAT; HELIX-LOOP-HELIX; VIRUS TYPE-I; HTLV-I; NOONAN-SYNDROME; GENE; ELEMENT; MYOGENESIS; CDNA;
Keywords:
myogenesis; basic leucine zipper; tax; tax response element; ribosomal protein L6;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Johnson, SE Penn State Univ, Dept Poultry Sci, 206 Henning, University Pk,PA 16802 USA Penn State Univ 206 Henning University Pk PA USA 16802 802 USA
Citazione:
S.R. Thomson e S.E. Johnson, "Isolation and characterization of chicken TaxREB107, a putative DNA binding protein abundantly expressed in muscle", GENE, 278(1-2), 2001, pp. 81-88

Abstract

Myogenic regulatory factors (MRFs) are vital transcription factors that act at multiple points during development to establish the skeletal muscle phenotype. This class of muscle-restricted, basic helix-loop-helix (bHLH) proteins acts in concert with additional transcriptional modulators to precisely control muscle gene expression. We have isolated the chicken homologue of Tax responsive element binding protein 107 (TaxREB107). The cDNA is 83% homologous at the amino acid level to human and mouse TaxREB107 and containsa centrally located leucine zipper motif. Northern analysis demonstrated that the gene is expressed in multiple tissues including skeletal muscle. Immunofluorescent staining revealed that the cTaxREB107 protein is located inboth the nuclear and cytoplasmic compartments. Distinct localization to the nucleoli supports the evidence that TaxREB107 is a ribosomal protein. Because TaxREB proteins also are implicated in transcriptional regulation, we overexpressed cTaxREB107 in embryonic myoblasts. cTaxREB107 increased troponin I reporter gene activity as well as MRF-directed transcription from a multimerized skeletal muscle E-box reporter gene (4Rtk-luc). However, cotransfection of expression plasmids coding for MyoD and cTaxREB107 did not produce an increase in 4Rtk-luc suggesting that cTaxREB107 enhances myogenic gene transcription through a means independent of a physical association withMyoD. In conclusion, our results define a role for cTaxREB107 during avianmyogenesis as a positive modulator of skeletal muscle gene expression. (C)2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 09:28:25