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Titolo:
Isolation and purification of chicken egg yolk immunoglobulins: A review
Autore:
De Meulenaer, B; Huyghebaert, A;
Indirizzi:
State Univ Ghent, Dept Food Technol & Nutr, Lab Food Chem & Anal, B-9000 Ghent, Belgium State Univ Ghent Ghent Belgium B-9000 Chem & Anal, B-9000 Ghent, Belgium
Titolo Testata:
FOOD AND AGRICULTURAL IMMUNOLOGY
fascicolo: 4, volume: 13, anno: 2001,
pagine: 275 - 288
SICI:
0954-0105(200112)13:4<275:IAPOCE>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENTEROTOXIGENIC ESCHERICHIA-COLI; LINKED-IMMUNOSORBENT-ASSAY; IMMUNIZED HENS; INTERACTION CHROMATOGRAPHY; ROTAVIRUS GASTROENTERITIS; PROTEIN FRACTIONATION; PASSIVE-IMMUNIZATION; 2-STEP PROCEDURE; IGY ANTIBODIES; SEPARATION;
Keywords:
Igy; isolation; purification; egg yolk; immunoglobulin;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
63
Recensione:
Indirizzi per estratti:
Indirizzo: De Meulenaer, B State Univ Ghent, Dept Food Technol & Nutr, Lab Food Chem & Anal, Coupure Links 653, B-9000 Ghent, Belgium State Univ Ghent Coupure Links 653 Ghent Belgium B-9000 m
Citazione:
B. De Meulenaer e A. Huyghebaert, "Isolation and purification of chicken egg yolk immunoglobulins: A review", FOOD AGR IM, 13(4), 2001, pp. 275-288

Abstract

Isolation and purification methods for immunoglobulins from hen egg yolk are reviewed. These methods consist of a removal of most of the lipoproteinsin order to obtain a water-soluble protein fraction, which can be regardedas an immunoglobulin concentrate. Water dilution methods and the use of particular anionic polysaccharides seem to offer the best IgY recoveries. Moreover both can be applied easily in an industrial environment. The further purification of the immunoglobulin can be achieved using precipitation, chromatographic or filtration methods. Filtration technology seems to offer the best opportunities for industrial applications while precipitation with polyethylene glycol or salts offer a cheap and easy methodology for laboratory use.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 11:53:23