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Titolo:
Stereological cell counts of GABAergic neurons in rat dentate hilus following transient cerebral ischemia
Autore:
Muller, GJ; Moller, A; Johansen, FF;
Indirizzi:
Univ Copenhagen, Neuropathol Lab, DK-2100 Copenhagen, Denmark Univ Copenhagen Copenhagen Denmark DK-2100 , DK-2100 Copenhagen, Denmark Neurosearch AS, DK-2750 Ballerup, Denmark Neurosearch AS Ballerup Denmark DK-2750 ch AS, DK-2750 Ballerup, Denmark
Titolo Testata:
EXPERIMENTAL BRAIN RESEARCH
fascicolo: 3, volume: 141, anno: 2001,
pagine: 380 - 388
SICI:
0014-4819(200112)141:3<380:SCCOGN>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUTAMIC-ACID DECARBOXYLASE; SOMATOSTATIN MESSENGER-RNA; HIPPOCAMPAL-FORMATION; SUBSTANTIA-NIGRA; TOTAL NUMBER; 2 FORMS; LOCALIZATION; PARVALBUMIN; REGION; GAD;
Keywords:
stereology; immunocytochemistry; Gad65/67; dentate hilus; ischemia; rat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Johansen, FF Univ Copenhagen, Neuropathol Lab, 11 Frederik Vs Vej, DK-2100Copenhagen, Denmark Univ Copenhagen 11 Frederik Vs Vej Copenhagen DenmarkDK-2100
Citazione:
G.J. Muller et al., "Stereological cell counts of GABAergic neurons in rat dentate hilus following transient cerebral ischemia", EXP BRAIN R, 141(3), 2001, pp. 380-388

Abstract

We have previously demonstrated a 60-80% ischemic loss of somatostatinergic neurons in the dorsal dentate hilus of the rat. However, several studies have failed to demonstrate ischemic loss of GABAergic neurons in hilus, although one study reports that 96% of the somatostatinergic neurons in the dorsal hilus colocalize GABA. In order to understand this paradox, we have now estimated, using unbiased stereology, the total number of neurons immunohistochemically stained against glutamic acid decarboxylase-65 (GAD65) and GAD67 in the dorsal dentate hilus. Rats were divided into groups subjected to either sham operation (n=8) or 8 min of transient global ischemia during systemical hypotension (n=8) and allowed to survive for 7-9 days. Results from cell counts (mean +/- SD) in sham rats demonstrated that the dorsal hilus contains 9,189 +/-3,957 GAD65 neurons and 6,991 +/-2,784 GAD67 neurons. After ischemia, corresponding cell counts demonstrated 10,216 +/-4,866 GAD65 neurons and 10,119 +/-5,906 GAD67 neurons, and these results were not significantly different (P >0.05) from results in sham rats. Power analysis ofthe t-test informs that losses less than 80% are not significant and reflects the excessive variance in our material. For comparison, we estimated a total of 21% ischemic neuron death in the dorsal hilus on cresyl violet-stained sections from other corresponding sham (n=7) and ischemic rats (n=7). This explains why ischemic loss of hilar GABAergic neurons can only be detected by counts of the vulnerable subpopulation colocalizing somatostatin. Our investigation has demonstrated a surprisingly high variation between rats in a number of GAD-immunopositive neurons located in the dorsal dentate hilus, which is related to variations between the individual rats and neurons in their endogenous GAD expression.

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Documento generato il 05/12/20 alle ore 01:11:18