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Titolo:
Genetic dissection of corticosterone receptor function in the rat hippocampus
Autore:
Vreugdenhil, E; de Kloet, ER; Schaaf, M; Datson, NA;
Indirizzi:
Leiden Amsterdam Ctr Drug Res, Div Med Pharmacol, NL-2300 RA Leiden, Netherlands Leiden Amsterdam Ctr Drug Res Leiden Netherlands NL-2300 RA Netherlands
Titolo Testata:
EUROPEAN NEUROPSYCHOPHARMACOLOGY
fascicolo: 6, volume: 11, anno: 2001,
pagine: 423 - 430
SICI:
0924-977X(200112)11:6<423:GDOCRF>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
CA2+/CALMODULIN-DEPENDENT PROTEIN-KINASE; GRANULE CELL DEGENERATION; HUMAN T-LYMPHOCYTES; GLUCOCORTICOID RECEPTOR; DIFFERENTIAL DISPLAY; TRANSCRIPTION FACTOR; MESSENGER-RNA; EXPRESSION; BRAIN; IV;
Keywords:
corticosterone; hippocampus; mineralocorticoid receptor; glucocorticoid receptor; expression profiling; differential display; SAGE; corticosterone-responsive genes; neuronal plasticity; CaMK;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Vreugdenhil, E Leiden Amsterdam Ctr Drug Res, Div Med Pharmacol, POB 9503,NL-2300 RA Leiden, Netherlands Leiden Amsterdam Ctr Drug Res POB 9503 Leiden Netherlands NL-2300 RA
Citazione:
E. Vreugdenhil et al., "Genetic dissection of corticosterone receptor function in the rat hippocampus", EUR NEUROPS, 11(6), 2001, pp. 423-430

Abstract

The hippocampus, a brain structure with a crucial role in learning and memory and an involvement in stress-related neurological or psychiatric disorders, is extremely sensitive to aberrant levels of corticosteroid stress hormones (CORT). We hypothesized that CORT-affected brain disorders are the result of aberrant expression of specific CORT-responsive genes. In order to identify such genes, we have applied several gene expression profiling techniques such as differential display, DNA micro-arrays and in particular thehighly sensitive serial analysis of gene expression (SAGE). Using SAGE, a total of 76,790 hippocampal tags were generated which together represent 28,748 unique mRNAs of which 4626 gave a hit with rat sequences in Genbank. By comparing SAGE profiles derived from rat hippocampi treated with different concentrations of corticosteroids, we have identified over 200 CORT-responsive genes with significant differential expression in hippocampus. The identified products include genes that are important for the plasticity of hippocampal neurones such as neural cell adhesion molecules, growth-promotingproteins, genes involved in axogenesis, synaptogenesis and signal-transduction. One novel corticosteroid-responsive gene, classified as Ca2+/calmodulin-dependent protein kinase (CaMK)-VI, exhibited structural resemblance with the family of CaMKs, in particular with that of CaMK-IV. We also identified an alternatively spliced mRNA of this gene encoding a peptide (CaMK-kinase related peptide or CARP) which may function in an autoregulatory feedback loop. These findings suggest a novel mode of operation of the CaMK pathway in control of Ca2+ homeostasis relevant for CORT-related brain disorders. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 19:38:12