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Titolo:
Roundup Ready (R) soybean event-specific real-time quantitative PCR assay and estimation of the practical detection and quantification limits in GMO analyses
Autore:
Berdal, KG; Holst-Jensen, A;
Indirizzi:
Natl Vet Inst, Sect Food & Feed Microbiol, N-0033 Oslo, Norway Natl Vet Inst Oslo Norway N-0033 d & Feed Microbiol, N-0033 Oslo, Norway
Titolo Testata:
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
fascicolo: 6, volume: 213, anno: 2001,
pagine: 432 - 438
SICI:
1438-2377(200111)213:6<432:RR(SER>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLUORESCENCE;
Keywords:
genetically modified organism; food analysis; roundup ready soy; event-specific PCR; detection and quantification limits;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Holst-Jensen, A Natl Vet Inst, Sect Food & Feed Microbiol, Ullevalsveien 68,POB 8156 Dep, N-0033 Oslo, Norway Natl Vet Inst Ullevalsveien 68,POB 8156Dep Oslo Norway N-0033
Citazione:
K.G. Berdal e A. Holst-Jensen, "Roundup Ready (R) soybean event-specific real-time quantitative PCR assay and estimation of the practical detection and quantification limits in GMO analyses", EUR FOOD RE, 213(6), 2001, pp. 432-438

Abstract

An event-specific real-time PCR method for detection and quantification ofgenetically modified Roundup Ready soybean with TaqMan chemistry on the LightCycler, targeting the nopaline synthase terminator (3 ') junction between recombinant and host plant DNA is described. We distinguish between threetypes of detection and quantification limits: the absolute limits (referring to the initial number of template copies in the PCR), the relative limits (referring to the relative percentage of initial template copies of the recombinant sequence to copies of the haploid soybean genome that is detected), and the practical limits (referring to what is applicable in the PCR with the DNA that is being analysed). The absolute detection limit was determined to be a single initial template copy, while the absolute quantification limit was determined to be approximately 30 initial template copies. We discuss the relative and practical limits, and provide guidelines to estimating the practical limits.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 07:24:06