Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Differential expression and tissue distribution of parkin isoforms during mouse development
Autore:
Huynh, DP; Dy, M; Nguyen, D; Kiehl, TR; Pulst, SM;
Indirizzi:
Univ Calif Los Angeles, Cedars Sinai Med Ctr, Sch Med, Div Neurol, Los Angeles, CA 90048 USA Univ Calif Los Angeles Los Angeles CA USA 90048 Los Angeles, CA 90048 USA Univ Calif Los Angeles, Cedars Sinai Med Ctr, Sch Med,Rose Moss Lab Parkinson & Neurodegenerat, Burns & Allen Res Inst, Los Angeles, CA 90048 USA Univ Calif Los Angeles Los Angeles CA USA 90048 Los Angeles, CA 90048 USA
Titolo Testata:
DEVELOPMENTAL BRAIN RESEARCH
fascicolo: 2, volume: 130, anno: 2001,
pagine: 173 - 181
SICI:
0165-3806(20011024)130:2<173:DEATDO>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECESSIVE JUVENILE PARKINSONISM; UBIQUITIN-PROTEIN LIGASE; ALPHA-SYNUCLEIN; LEWY BODIES; RAT-BRAIN; DISEASE; GENE; CLONING; IDENTIFICATION; MUTATIONS;
Keywords:
parkin; early onset autosomal recessive juvenile Parkinson disease; parkin expression; mouse embryo;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Pulst, SM Univ Calif Los Angeles, Cedars Sinai Med Ctr, Sch Med, Div Neurol, 8700 Beverley Blvd, Los Angeles, CA 90048 USA Univ Calif Los Angeles 8700 Beverley Blvd Los Angeles CA USA 90048
Citazione:
D.P. Huynh et al., "Differential expression and tissue distribution of parkin isoforms during mouse development", DEV BRAIN R, 130(2), 2001, pp. 173-181

Abstract

Mutations of the park-in gene are a cause of autosomal recessive juvenile parkinsonism. Although the parkin gene has been isolated from mouse, rat, and human, little is known about its expression in neural and nonneural tissues during development. In this study, we used a polyclonal antibody to a peptide downstream of the park-in ubiquitin domain to investigate (1) the differential expression of parkin isoforms in protein extracts from fetal andadult mouse tissues, and (2) the distribution of parkin in mouse fetal tissues at different developmental stages and in adult CNS tissues. By Westernblot analyses, at least three isoforms of parkin of 22, 50, and 55 kDa were differentially expressed in mouse tissues. The p22 and p50 isoforms were found in fetal and adult mouse CNS tissues, while the p55 isoform was foundonly in adult tissues. The p50 isoform is the predominant form in both fetal and adult tissues. Immunolocalization in mouse fetuses showed that parkin was expressed only after neuronal differentiation. Although parkin was localized throughout the cytoplasm, the highest level of parkin was found in the neurites of both fetal and adult neurons. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 12:11:27