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Titolo:
Increase of cGMP, cADP-ribose and inositol 1,4,5-trisphosphate preceding Ca2+ transients in fertilization of sea urchin eggs
Autore:
Kuroda, R; Kontani, K; Kanda, Y; Katada, T; Nakano, T; Satoh-, Y; Suzuki, N; Kuroda, H;
Indirizzi:
Toyama Univ, Fac Sci, Dept Environm Biol & Chem, Toyama 9308555, Japan Toyama Univ Toyama Japan 9308555 ronm Biol & Chem, Toyama 9308555, Japan Univ Tokyo, Grad Sch Pharmaceut Sci, Dept Physiol Sci, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 siol Sci, Bunkyo Ku, Tokyo 1130033, Japan Hokkaido Univ, Grad Sch Sci, Div Biol Sci, Kita Ku, Sapporo, Hokkaido 0600810, Japan Hokkaido Univ Sapporo Hokkaido Japan 0600810 oro, Hokkaido 0600810, Japan
Titolo Testata:
DEVELOPMENT
fascicolo: 22, volume: 128, anno: 2001,
pagine: 4405 - 4414
SICI:
0950-1991(200111)128:22<4405:IOCCAI>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLIC ADP-RIBOSE; INDUCED CALCIUM-RELEASE; PHOSPHOLIPASE C-GAMMA; INTRACELLULAR CA2+; NITRIC-OXIDE; RYANODINE RECEPTORS; GUANYLATE-CYCLASE; SIGNALING PATHWAY; XENOPUS-LAEVIS; BETA-CELLS;
Keywords:
cGMP; cADP-ribose; IP3; Ca2+ transient; fertilization; sea urchin eggs;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Kuroda, H Toyama Univ, Fac Sci, Dept Environm Biol & Chem, 3190 Gofuku, Toyama 9308555, Japan Toyama Univ 3190 Gofuku Toyama Japan 9308555 ama 9308555, Japan
Citazione:
R. Kuroda et al., "Increase of cGMP, cADP-ribose and inositol 1,4,5-trisphosphate preceding Ca2+ transients in fertilization of sea urchin eggs", DEVELOPMENT, 128(22), 2001, pp. 4405-4414

Abstract

Transient increases, or oscillations, of cytoplasmic free Ca2+ concentration, [Ca2+](i), occur during fertilization of animal egg cells. In sea urchin eggs, the increased Ca2+ is derived from intracellular stores, but the principal signaling and release system involved has not yet been agreed upon. Possible candidates are the inositol 1,4,5-trisphosphate receptor/channel (IP3R) and the ryanodine receptor/channel (RyR) which is activated by cGMP or cyclic ADP-ribose (cADPR). Thus, it seemed that direct measurements of the likely second messenger candidates during sea urchin fertilization wouldbe essential to an understanding of the Ca2+ signaling pathway. We therefore measured the cGMP, cADPR and inositol 1,4,5-trisphosphate (IP3) contentsof sea urchin eggs during the early stages of fertilization and compared these with the [Ca2+](i) rise in the presence or absence of an inhibitor against soluble guanylate cyclase. We obtained three major experimental results: (1) cytosolic cGMP levels began to rise first, followed by cADPR and IP3levels, all almost doubling before the explosive increase of [Ca2+](i); (2) most of the rise in IP3 occurred after the Ca2+ peak; IP3 production could also be induced by the artificial elevation of [Ca2+](i), suggesting the large increase in IP3 is a consequence, rather than a cause, of the Ca2+ transient; (3) the measured increase in cGMP was produced by the soluble guanylate cyclase of eggs, and inhibition of soluble guanylate cyclase of eggs diminished the production of both cADPR and IP3 and the [Ca2+](i) increase without the delay of Ca2+ transients. Taken together, these results suggestthat the RyR pathway involving cGMP and cADPR is not solely responsible for the initiating event, but contributes to the Ca2+ transients by stimulating IP3 production during fertilization of sea urchin eggs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 09:46:10