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Titolo:
Molecular detection of rifampin and ofloxacin resistance for patients who experience relapse of multibacillary leprosy
Autore:
Cambau, E; Bonnafous, P; Perani, E; Sougakoff, W; Ji, BH; Jarlier, V;
Indirizzi:
Univ Paris 06, Lab Bacteriol Hyg, Ctr Natl Reference Surveillance Infect Mycobacter, F-75634 Paris 13, France Univ Paris 06 Paris France 13 nfect Mycobacter, F-75634 Paris 13, France
Titolo Testata:
CLINICAL INFECTIOUS DISEASES
fascicolo: 1, volume: 34, anno: 2002,
pagine: 39 - 45
SICI:
1058-4838(20020101)34:1<39:MDORAO>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONFORMATION POLYMORPHISM ANALYSIS; MYCOBACTERIUM-LEPRAE; DRUG-RESISTANCE; RPOB GENE; TUBERCULOSIS; DAPSONE; QUINOLONES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Cambau, E Univ Paris 06, Lab Bacteriol Hyg, Ctr Natl Reference Surveillance Infect Mycobacter, 91 Blvd Hop, F-75634 Paris 13, France Univ Paris 06 91Blvd Hop Paris France 13 5634 Paris 13, France
Citazione:
E. Cambau et al., "Molecular detection of rifampin and ofloxacin resistance for patients who experience relapse of multibacillary leprosy", CLIN INF D, 34(1), 2002, pp. 39-45

Abstract

Molecular detection of rifampin resistance (rpoB analysis) in Mycobacterium leprae was determined for 49 patients who experienced relapse of multibacillary leprosy and for 34 untreated patients. Molecular detection of ofloxacin resistance (gyrA analysis) was determined for the 12 patients who experienced relapse and who had received ofloxacin. Results of molecular tests were compared with the reference susceptibility test in the mouse footpad. Overall, the efficiency of molecular detection-that is, positive DNA amplification-was 95%, whereas that of the in vivo test was 55% (P < .001). Results of molecular detection and in vivo test were fully concordant when both were available-that is, for 35 rifampin-sensitive cases of leprosy (no rpoB mutation), 4 ofloxacin-sensitive cases (no gyrA mutation), 11 rifampin-resistant cases (rpoB missense mutations), and 1 ofloxacin-resistant case (gyrAmutation). rpoB and gyrA analysis appears to be an effective method for detection of rifampin and ofloxacin resistance in patients with leprosy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 19:43:57