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Titolo:
In vitro effects of cholesteryl butyrate solid lipid nanospheres as a butyric acid pro-drug on melanoma cells: Evaluation of antiproliferative activity and apoptosis induction
Autore:
Salomone, B; Ponti, R; Gasco, MR; Ugazio, E; Quaglino, P; Osella-Abate, S; Bernengo, MG;
Indirizzi:
Univ Turin, Sect Dermatol 1, Dept Med & Surg Specialties, I-10126 Turin, Italy Univ Turin Turin Italy I-10126 & Surg Specialties, I-10126 Turin, Italy Univ Turin, Dept Sci & Tecnol Farm, I-10126 Turin, Italy Univ Turin Turin Italy I-10126 t Sci & Tecnol Farm, I-10126 Turin, Italy
Titolo Testata:
CLINICAL & EXPERIMENTAL METASTASIS
fascicolo: 8, volume: 18, anno: 2001,
pagine: 663 - 673
SICI:
0262-0898(2001)18:8<663:IVEOCB>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
HISTONE DEACETYLASE INHIBITORS; BREAST-CANCER CELLS; CHAIN FATTY-ACIDS; SODIUM-BUTYRATE; ANTICANCER PRODRUGS; ARGININE BUTYRATE; EPITHELIAL-CELLS; BCL-2 EXPRESSION; GENE-EXPRESSION; CARCINOMA-CELLS;
Keywords:
apoptosis; butyrate; cell cycle; cholesteryl butyrate; drug delivery; melanoma; solid lipid nanospheres;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
66
Recensione:
Indirizzi per estratti:
Indirizzo: Bernengo, MG Univ Turin, Sect Dermatol 1, Dept Med & Surg Specialties, ViaCherasco 23,I-10126 Turin, Italy Univ Turin Via Cherasco 23 Turin Italy I-10126 Turin, Italy
Citazione:
B. Salomone et al., "In vitro effects of cholesteryl butyrate solid lipid nanospheres as a butyric acid pro-drug on melanoma cells: Evaluation of antiproliferative activity and apoptosis induction", CLIN EXP M, 18(8), 2001, pp. 663-673

Abstract

Literature data show that butyric acid derivatives bear a dose-dependent differentiative anti-proliferative activity on cancer cell lines and that apoptosis induction may play a major role. Although it was recently shown that solid lipid nanospheres (SLNs) are a suitable tool for several in vivo drug administration routes, there is little available information on melanomacell lines. This study was aimed at evaluating the anti-proliferative and apoptotic in vitro effects of cholesteryl butyrate (chol-but) SLNs on melanoma cells. Increasing concentrations of chol-but SLNs were used to test twomelanoma cell lines. Both cell lines were treated with Na-butyrate (Na-but) and chol-but SLNs for viability. Those tested with chol-but SLNs were more effective than Na-butirate (3 to 72 h). The apoptotic effects of chol-butSLNs were evaluated between 3 and 72 h by annexin-V (ANX-V)/propidium iodide (PI) staining and the antiproliferative effect by PI staining. Apoptosisanti-proliferative-regulatory proteins as bcl-2, Fas/APO1 (CD95) and PCNA (PC10) were also investigated. Flow cytometric analyses evidenced a G(0/1)-S transition block and a `sub-G(0/1)' apoptotic peak from 0.5 to 1.0 mM butyric acid. In ANX-V/PI flow cytometric staining, a dose- and time-dependentincrease in the apoptotic cell percentage (ANX-V+) coupled with a down-regulation of PC10 and bcl-2 and a parallel up-regulation of Fas/APO1 (CD95) were found in both lines started after 3 to 24 h of chol-but SLNs treatment. Results show that chol-but SLNs exerts a dose/time-dependent effect in melanoma cell apoptosis induction between 3 and 24 h and a dose but not time-dependent effect after 24 h of treatment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 19:54:45