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Titolo:
Regulation of calcium sparks and spontaneous transient outward currents byRyR3 in arterial vascular smooth muscle cells
Autore:
Lohn, M; Jessner, W; Furstenau, M; Wellner, M; Sorrentino, V; Haller, H; Luft, FC; Gollasch, M;
Indirizzi:
Humboldt Univ, Fac Med Charite, Franz Volhard Clin, HELIOS Klinikum Berlin, D-13125 Berlin, Germany Humboldt Univ Berlin Germany D-13125 kum Berlin, D-13125 Berlin, Germany Humboldt Univ, Fac Med Charite, Max Delbruck Ctr Mol Med, D-13125 Berlin, Germany Humboldt Univ Berlin Germany D-13125 tr Mol Med, D-13125 Berlin, Germany Univ Vienna, Sch Med, Dept Pathophysiol, A-1010 Vienna, Austria Univ Vienna Vienna Austria A-1010 t Pathophysiol, A-1010 Vienna, Austria San Raffaele Sci Inst, DIBIT, I-20132 Milan, Italy San Raffaele Sci Inst Milan Italy I-20132 t, DIBIT, I-20132 Milan, Italy Univ Siena, Dept Neurosci, Mol Med Sect, I-53100 Siena, Italy Univ Siena Siena Italy I-53100 rosci, Mol Med Sect, I-53100 Siena, Italy Hannover Med Sch, Dept Nephrol, D-3000 Hannover, Germany Hannover Med SchHannover Germany D-3000 phrol, D-3000 Hannover, Germany
Titolo Testata:
CIRCULATION RESEARCH
fascicolo: 11, volume: 89, anno: 2001,
pagine: 1051 - 1057
SICI:
0009-7330(20011123)89:11<1051:ROCSAS>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
CA2+ RELEASE CHANNELS; SKELETAL-MUSCLE; CORONARY-ARTERY; RYANODINE RECEPTOR-TYPE-3; CEREBRAL-ARTERIES; BETA-1 SUBUNIT; CONTRACTION; EXPRESSION; MYOCYTES; REQUIREMENT;
Keywords:
potassium currents; membrane potentials; caveolae; sarcoplasmic reticulum; ryanodine receptor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Gollasch, M Humboldt Univ, Fac Med Charite, Franz Volhard Clin, HELIOS Klinikum Berlin, Wiltbergstr 50, D-13125 Berlin, Germany Humboldt Univ Wiltbergstr 50 Berlin Germany D-13125 , Germany
Citazione:
M. Lohn et al., "Regulation of calcium sparks and spontaneous transient outward currents byRyR3 in arterial vascular smooth muscle cells", CIRCUL RES, 89(11), 2001, pp. 1051-1057

Abstract

Intracellular Ca2+ levels control both contraction and relaxation in vascular smooth muscle cells (VSMCs). Ca2+-dependent relaxation is mediated by discretely localized Ca2+ release events through ryanodine receptor (RyR) channels in the sarcoplasmic reticulum (SR). These local increases in Ca2+ concentration, termed sparks, stimulate nearby Ca2+-activated K+ (BK) channels causing BK currents (spontaneous transient outward currents or STOCs). STOCs are hyperpolarizing currents that oppose vasoconstriction. Several RyR isoforms are coexpressed in VSMCs; however, their role in Ca2+ spark generation is unknown. To provide molecular information on RyR cluster function and assembly, we examined Ca2+ sparks and STOCs in RyR3-deficient freshly isolated myocytes of resistance-sized cerebral arteries from knockout mice and compared them to Ca2+ sparks in cells from wild-type mice. We used RT-PCRto identify RyR1, RyR2, and RyR3 mRNA in cerebral arteries. Ca2+. sparks in RyR3-deficient cells were similar in peak amplitude (measured as F/F-o), width at half-maximal amplitude, and duration compared with wild-type cell Ca2+ sparks. However, the frequency of STOCs (between -60 mV and -20 mV) was significantly higher in RyR3-deficient cells than in wild-type cells. Ca2 sparks and STOCs in both RyR3-deficient and wild-type cells were inhibited by ryanodine (10 mu mol/L), external Ca2+ removal, and depletion of SR Ca2+ stores by caffeine (1 mmol/L), Isolated, pressurized cerebral arteries of RyR3-deficient mice developed reduced myogenic tone. Our results suggest that RyR3 is part of the SR Ca2+ spark release unit and plays a specific molecular role in the regulation of STOCs frequency in mouse cerebral artery VSMCs after decreased arterial tone.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 03:10:45