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Titolo:
A rapid screening method for a single nucleotide polymorphism (SNP) in thehuman MOR gene
Autore:
Grosch, S; Niederberger, E; Lotsch, J; Skarke, C; Geisslinger, G;
Indirizzi:
Univ Frankfurt Klinikum, Inst Klin Pharmakol, Pharmazentrum Frankfurt, D-60590 Frankfurt, Germany Univ Frankfurt Klinikum Frankfurt Germany D-60590590 Frankfurt, Germany
Titolo Testata:
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
fascicolo: 6, volume: 52, anno: 2001,
pagine: 711 - 714
SICI:
0306-5251(200112)52:6<711:ARSMFA>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
MU-OPIOID RECEPTOR; ALCOHOL DEPENDENCE; OPIATE RECEPTOR; CANDIDATE GENE; VARIANTS; ASSOCIATION; SENSITIVITY; NOCICEPTION; EXPRESSION;
Keywords:
mu opioid receptor (MOR); fluorescence resonance energy transfer (FRET); LightCycler; polymorphism; SNP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Geisslinger, G Univ Frankfurt Klinikum, Inst Klin Pharmakol, PharmazentrumFrankfurt, Theodor Stern Kai 7, D-60590 Frankfurt, Germany Univ Frankfurt Klinikum Theodor Stern Kai 7 Frankfurt Germany D-60590
Citazione:
S. Grosch et al., "A rapid screening method for a single nucleotide polymorphism (SNP) in thehuman MOR gene", BR J CL PH, 52(6), 2001, pp. 711-714

Abstract

Aims Genetic association Studies have suggested chat the single nucleotidepolymorphism (SNP) at position 118 of the human mu -opioid receptor (MOR) gene could be a potential risk Factor For drug treatment variability in patients. Therefore, we wanted to develop a fast and reliable detection methodfor this SNP which is applicable in a clinical setting. Methods To detect the polymorphism at position A118-->G in the human MOR, gene we used the fluorescence resonance energy transfer (FRET)-PCR technique with subsequent melting curve analysis. Results The polymorphism at position A118-->G in the human MOR gene could be clearly discriminated with melting peak temperatures of 69.8 degrees C and 63.8 degrees C, corresponding to the wild type and mutated MOR allele, respectively. The results from FRET-PCR were validated by sequencing and restriction-fragment length polymorphism (RFLP). Screening of blood samples from 100 subjects showed an allelic distribution for the human MOR alleles of79% homozygous wild type). 20% (heterozygous) and 0.9% (homozygous mutated). Conclusions The FRET-PCR protocol for detection of the human MOR gene polymorphism at position 118 offers a rapid and reliable method which could be used for population screening of this and other genes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 15:00:38