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Titolo:
Fluorescence in situ hybridization (FISH) in diagnostic and investigative neuropathology
Autore:
Fuller, CE; Perry, A;
Indirizzi:
Washington Univ, Sch Med, Div Neuropathol, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 Neuropathol, St Louis, MO 63110 USA
Titolo Testata:
BRAIN PATHOLOGY
fascicolo: 1, volume: 12, anno: 2002,
pagine: 67 - 86
SICI:
1015-6305(200201)12:1<67:FISH(I>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; PRIMITIVE NEUROECTODERMAL TUMORS; COMPARATIVE GENOMIC HYBRIDIZATION; MOLECULAR-GENETIC-ANALYSIS; PARAFFIN-EMBEDDED TISSUE; N-MYC AMPLIFICATION; PEDIATRIC-ONCOLOGY-GROUP; HIGH-GRADE ASTROCYTOMAS; CHROMOSOME ARM 17P; NF1 GENE;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
203
Recensione:
Indirizzi per estratti:
Indirizzo: Perry, A Washington Univ, Sch Med, Div Neuropathol, Box 8118,660 S Euclid Ave, St Louis, MO 63110 USA Washington Univ Box 8118,660 S Euclid Ave St Louis MO USA 63110 A
Citazione:
C.E. Fuller e A. Perry, "Fluorescence in situ hybridization (FISH) in diagnostic and investigative neuropathology", BRAIN PATH, 12(1), 2002, pp. 67-86

Abstract

Over the last decade, fluorescence in situ hybridization (FISH) has emerged as a powerful clinical and research tool for the assessment of target DNAdosages within interphase nuclei. Detectable alterations include aneusomies, deletions, gene amplifications, and translocations, with primary advantages to the pathologist including its basis in morphology, its applicabilityto archival, formalin-fixed paraffin-embedded (FFPE) material, and its similarities to Immunohistochemistry. Recent technical advances such as Improved hybridization protocols, markedly expanded probe availability resulting from the human genome sequencing initiative, and the advent of high-throughput assays such as gene chip and tissue microarrays have greatly enhanced the applicability of FISH. In our lab, we currently utilize only a limited battery of DNA probes for routine diagnostic purposes, with determination ofchromosome 1p and 19q dosage in oligodendroglial neoplasms representing the most common application. However, research applications are numerous and will likely translate into a growing list of clinically useful markers in the near future. In this review, we highlight the advantages and disadvantages of FISH and familiarize the reader with current applications In diagnostic and investigative neuropathology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 07:00:19