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Titolo:
Real-time quantitative polymerase chain reaction: A potential tool for genetic analysis in neuropathology
Autore:
Aldape, K; Ginzinger, DG; Godfrey, TE;
Indirizzi:
Univ Texas, MD Anderson Canc Ctr, Dept Pathol, Neuropathol Sect, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 , Neuropathol Sect, Houston, TX 77030 USA Univ Calif San Francisco, Ctr Comprehens Canc, San Francisco, CA 94143 USAUniv Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Pittsburgh, Inst Canc, Dept Surg, Pittsburgh, PA 15260 USA Univ Pittsburgh Pittsburgh PA USA 15260 pt Surg, Pittsburgh, PA 15260 USA Univ Pittsburgh, Inst Canc, Dept Human Genet, Pittsburgh, PA 15260 USA Univ Pittsburgh Pittsburgh PA USA 15260 n Genet, Pittsburgh, PA 15260 USA
Titolo Testata:
BRAIN PATHOLOGY
fascicolo: 1, volume: 12, anno: 2002,
pagine: 54 - 66
SICI:
1015-6305(200201)12:1<54:RQPCRA>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
CANCER-PATIENTS; MESSENGER-RNA; CEREBROSPINAL-FLUID; MOLECULAR BEACONS; POINT MUTATIONS; DYSTROPHIN GENE; BREAST-CANCER; BRAIN-TUMORS; SERUM DNA; DIAGNOSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
67
Recensione:
Indirizzi per estratti:
Indirizzo: Aldape, K Univ Texas, MD Anderson Canc Ctr, Dept Pathol, Neuropathol Sect,Box 85,1515 Holcombe Blvd, Houston, TX 77030 USA Univ Texas Box 85,1515 Holcombe Blvd Houston TX USA 77030 30 USA
Citazione:
K. Aldape et al., "Real-time quantitative polymerase chain reaction: A potential tool for genetic analysis in neuropathology", BRAIN PATH, 12(1), 2002, pp. 54-66

Abstract

Since its introduction in the early- to mid-1980s, the polymerase chain reaction (PCR) has been modified and optimized for an increasing number of applications. Early on, the focus was on the amplification of a specific nucleic acid template into quantities amenable to identification and experimental manipulation. While this remains an important application, recent technology has allowed the use of PCR to accurately quantitate the amount of a specific nucleic acid template present in a complex sample. Rather than simply analyzing the final product amount following the course of sequential cycles of amplification, quantitative PCR allows one to measure the accumulation of PCR product during the course of the reaction ("real-time PCR"). Under the appropriate conditions the number of PCR cycles required for the accumulation of a specific amount of product (during the exponential phase of the reaction) is a reflection of the relative amount of nucleic acid template present in the sample under analysis. Real-time quantitative PCR allows one to analyze a relatively large number of samples in a short period of time, potentially allowing multiple markers to be applied on a sample within atime frame consistent with clinical settings. In this overview, we will highlight the uses of real-time quantitative PCR as a potential diagnostic tool in neuropathology, focusing on the analysis of CNS tumors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 09:33:42