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Titolo:
Immunohistochemistry for the prion protein: Comparison of different monoclonal antibodies in human prion disease subtypes
Autore:
Kovacs, GG; Head, MW; Hegyi, I; Bunn, TJ; Flicker, H; Hainfellner, JA; McCardle, L; Laszlo, L; Jarius, C; Ironside, JW; Budka, H;
Indirizzi:
Univ Vienna, Inst Neurol, A-1097 Vienna, Austria Univ Vienna Vienna Austria A-1097 a, Inst Neurol, A-1097 Vienna, Austria Austrian Reference Ctr Human Prion Dis, Vienna, Austria Austrian ReferenceCtr Human Prion Dis Vienna Austria , Vienna, Austria Semmelweis Univ, Dept Neurol, Budapest, Hungary Semmelweis Univ BudapestHungary s Univ, Dept Neurol, Budapest, Hungary Univ Edinburgh, Western Gen Hosp, Natl CJD Surveillance Unit, Edinburgh, Midlothian, Scotland Univ Edinburgh Edinburgh Midlothian Scotland burgh, Midlothian, Scotland Univ Edinburgh, Western Gen Hosp, Dept Pathol, Edinburgh, Midlothian, Scotland Univ Edinburgh Edinburgh Midlothian Scotland burgh, Midlothian, Scotland Inst Neuropathol, Dept Pathol, Zurich, Switzerland Inst Neuropathol Zurich Switzerland l, Dept Pathol, Zurich, Switzerland Eotvos Univ Sci, Dept Gen Zool, Budapest, Hungary Eotvos Univ Sci Budapest Hungary Sci, Dept Gen Zool, Budapest, Hungary
Titolo Testata:
BRAIN PATHOLOGY
fascicolo: 1, volume: 12, anno: 2002,
pagine: 1 - 11
SICI:
1015-6305(200201)12:1<1:IFTPPC>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
CREUTZFELDT-JAKOB-DISEASE; SPONGIFORM ENCEPHALOPATHIES; VARIANT; BRAIN; CLASSIFICATION; CODON-129; EPITOPE; TISSUE; PRPSC; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Budka, H Univ Vienna, Inst Neurol, AKH 4J,Wahringer Gurtel 18-20, A-1097 Vienna, Austria Univ Vienna AKH 4J,Wahringer Gurtel 18-20 Vienna Austria A-1097
Citazione:
G.G. Kovacs et al., "Immunohistochemistry for the prion protein: Comparison of different monoclonal antibodies in human prion disease subtypes", BRAIN PATH, 12(1), 2002, pp. 1-11

Abstract

Demonstration of the abnormal form of the prion protein (PrP) in the brainconfirms the diagnosis of human prion disease (PrD). Using immunohistochemistry, we have compared ten monoclonal antibodies In PrD subtypes Includingsporadic and variant Creutzfeldt-Jakob disease (CJD), fatal familial insomnia, Alzheimer's disease (AD), and control brains. CJD subgroups were determined using Western blot analysis for the protease-resistant PrP type In combination with sequencing to determine the genotype at the methionine/valine polymorphism at codon 129 of the prion protein gene. None of the antibodies labeled given subgroups exclusively, but the Intensity of immunoreactivity varied among morphologically distinct types of deposit. Fine granular orsynaptic PrP deposits stained weakly or not at all with antibodies againstthe N-terminus of PrP, and were visible in one case only with 12F10 and SAF54. Coarser and plaque type deposits were immunolabeled with all antibodies. The immunostaining patterns appear characteristic for the disease subgroups. Labeling of certain neurons In all cases irrespective of disease, and staining at the periphery and/or throughout the senile plaques of AD patients were also noted. Antibodies such as 6H4 and 12F10 failed to give this type of labeling and are therefore less likely to recognise non-pathological PrP material in immunohistochemistry.

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Documento generato il 03/04/20 alle ore 07:11:07