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Titolo:
Oxygenation reactions of various tricyclic fused aromatic compounds using Escherichia coli and Streptomyces lividans transformants carrying several arene dioxygenase genes
Autore:
Shindo, K; Ohnishi, Y; Chun, HK; Takahashi, H; Hayashi, M; Saito, A; Iguchi, K; Furukawa, K; Harayama, S; Horinouchi, S; Misawa, N;
Indirizzi:
Kirin Brewery Co Ltd, Cent Labs Key Technol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan Kirin Brewery Co Ltd Yokohama Kanagawa Japan 2360004 agawa 2360004, Japan Kirin Brewery Co Ltd, Pharmaceut Res Labs, Takasaki, Gumma 3701295, Japan Kirin Brewery Co Ltd Takasaki Gumma Japan 3701295 i, Gumma 3701295, Japan Univ Tokyo, Grad Sch Agr & Life Sci, Dept Biotechnol, Bunkyo Ku, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 otechnol, Bunkyo Ku, Tokyo 1138657, Japan Tokyo Univ Pharm & Life Sci, Tokyo 1920392, Japan Tokyo Univ Pharm & Life Sci Tokyo Japan 1920392 ci, Tokyo 1920392, Japan Marine Biotechnol Inst, Heita, Kamaishi 0260001, Japan Marine Biotechnol Inst Heita Kamaishi Japan 0260001 maishi 0260001, Japan Kyushu Univ, Div Bioresource & Bioenvironm Sci, Fukuoka 8128581, Japan Kyushu Univ Fukuoka Japan 8128581 ioenvironm Sci, Fukuoka 8128581, Japan
Titolo Testata:
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
fascicolo: 11, volume: 65, anno: 2001,
pagine: 2472 - 2481
SICI:
0916-8451(200111)65:11<2472:OROVTF>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
PSEUDOMONAS-PUTIDA F1; ABSOLUTE-CONFIGURATIONS; TOLUENE DEGRADATION; EVOLUTIONARY RELATIONSHIP; NAPHTHALENE DIOXYGENASE; SUBSTRATE-SPECIFICITY; EFFICIENT DEGRADATION; ENCODING BIPHENYL; TOD OPERON; EXPRESSION;
Keywords:
tricyclic aromatic compounds; phenanthridine; arene dioxygenase; Escherichia coli; Streptomyces lividans;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Misawa, N Kirin Brewery Co Ltd, Cent Labs Key Technol, Kanazawa Ku, 1-13-5Fukuura, Yokohama, Kanagawa 2360004, Japan Kirin Brewery Co Ltd 1-13-5 Fukuura Yokohama Kanagawa Japan 2360004
Citazione:
K. Shindo et al., "Oxygenation reactions of various tricyclic fused aromatic compounds using Escherichia coli and Streptomyces lividans transformants carrying several arene dioxygenase genes", BIOS BIOT B, 65(11), 2001, pp. 2472-2481

Abstract

Bioconversion (biotransformation) experiments on arenes (aromatic compounds), including various tricyclic fused aromatic compounds such as fluorene, dibenzofuran, dibenzothiophene, carbazole, acridene, and phenanthridine, were done using the cells of Escherichia coli transformants expressing several arene dioxygenase genes. E. coli carrying the phenanthrene dioxygenase (phdABCD) genes derived from the marine bacterium Nocardioides sp. strain KP7converted all of these tricyclic aromatic compounds, while E. coli carrying the Pseudomonas putida F1 toluene dioxygenase (todC1C2BA) genes or the P.pseudoalcaligenes KF707 biphenyl dioxygenase (bphA1A2A3A4) genes was not able to convert these substrates. Surprisingly, E. coli carrying hybrid dioxygenase (todC1::bphA2A3A4) genes with a subunit substitution between the toluene and biphenyl dioxygenases was able to convert fluorene, dibenzofuran,and dibenzothiophene. The cells of a Streptomyces lividans transformant carrying the phenanthrene dioxygenase genes were also evaluated for bioconversion of various tricyclic fused aromatic compounds. The ability of this actinomycete in their conversion was similar to that of E. coli carrying the corresponding genes. Products converted from the aromatic compounds with these recombinant bacterial cells were purified by column chromatography on silica gel, and identified by their MS and H-1 and C-13 NMR analyses. Severalproducts, e.g., 4-hdroxyfluorene converted from fluorene, and cis-1,2-dihydroxy-1,2-dihydrophenanthridine, cis-9,10-dihydroxy-9,10-dihydrophenanthridine, and 10-hydroxyphenanthridine, which were converted from phenanthridine, were novel compounds.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 10:59:12