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Titolo:
Two pathways of iron uptake in bovine spleen apoferritin dependent on ironconcentration
Autore:
Orino, K; Kamura, S; Natsuhori, M; Yamamoto, S; Watanabe, K;
Indirizzi:
Kitasato Univ, Sch Vet Med & Anim Sci, Biochem Lab, Towada, Aomori 0348628, Japan Kitasato Univ Towada Aomori Japan 0348628 , Towada, Aomori 0348628, Japan Kitasato Univ, Sch Vet Med & Anim Sci, Lab Vet Radiol, Towada, Aomori 0348628, Japan Kitasato Univ Towada Aomori Japan 0348628 , Towada, Aomori 0348628, Japan
Titolo Testata:
BIOMETALS
fascicolo: 1, volume: 15, anno: 2001,
pagine: 59 - 63
SICI:
0966-0844(2001)15:1<59:TPOIUI>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
HORSE SPLEEN; L-CHAIN; HUMAN FERRITIN; H-CHAIN; PHOSPHATE; MECHANISM; HETEROPOLYMERS; INDUCTION; CELLS; POOL;
Keywords:
ferritin; ferroxidase; iron; iron uptake; kinetics;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Orino, K Kitasato Univ, Sch Vet Med & Anim Sci, Biochem Lab, Towada, Aomori 0348628, Japan Kitasato Univ Towada Aomori Japan 0348628 Aomori 0348628, Japan
Citazione:
K. Orino et al., "Two pathways of iron uptake in bovine spleen apoferritin dependent on ironconcentration", BIOMETALS, 15(1), 2001, pp. 59-63

Abstract

Iron incorporation by bovine spleen apoferritin either with ferrous ammonium sulfate in different buffers or with ferrous ammonium sulfate and phosphate was studied. Iron uptake and iron autoxidation were recorded spectrophotomerically. The buffers [4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid (Hepes) and tris(hydroxymethyl)aminoethane (Tris) exhibited pH-dependent iron autoxidation, with Tris showing less iron autoxidation than Hepes. An Eadie-Scatchard plot (v/[s] versus v) of the iron uptake rate in Hepes was a curved rather than a straight line, suggesting that there are two ironuptake pathways. On the other hand, the Eadie-Scatchard plots of Tris and of Hepes after the addition of phosphate showed a straight line. Phosphate accelerated the iron uptake rate. The iron loading kinetics of apoferritin in Hepes was dependent on apoferritin concentration. The K-m value obtainedfrom iron uptake kinetics was 4.5 muM, corresponding to the physiological iron concentration. These results demonstrate that iron loading of apoferritin was accomplished at physiological iron concentrations, which is essential for iron uptake, via two uptake pathways of dependent on iron concentration.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 22:39:44