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Titolo:
pH dependent conformational changes within the iron responsive element
Autore:
Meehan, HA; Connell, GJ;
Indirizzi:
Univ Minnesota, Dept Pharmacol, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 rmacol, Minneapolis, MN 55455 USA
Titolo Testata:
BIOMETALS
fascicolo: 1, volume: 15, anno: 2001,
pagine: 27 - 32
SICI:
0966-0844(2001)15:1<27:PDCCWT>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
FERRITIN MESSENGER-RNA; BINDING-PROTEIN; TRANSFERRIN RECEPTOR; TRANSLATIONAL REGULATION; ACONITASE ACTIVITY; HIGH-AFFINITY; SEQUENCE; REGION; NMR; IDENTIFICATION;
Keywords:
dimethylsulphate modification interference; IRE; iron homeostasis; perturbed pK(a); RNA structure;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Connell, GJ Univ Minnesota, Dept Pharmacol, 3-249 Millard Hall, Minneapolis, MN 55455 USA Univ Minnesota 3-249 Millard Hall Minneapolis MN USA 55455 USA
Citazione:
H.A. Meehan e G.J. Connell, "pH dependent conformational changes within the iron responsive element", BIOMETALS, 15(1), 2001, pp. 27-32

Abstract

The expression of several mRNAs related to iron importation, storage and utilization within mammalian cells are regulated through interactions of iron regulatory proteins with an iron responsive element, an RNA hairpin with a bulged C. A dimethylsulfate modification interference assay was used to demonstrate that the iron responsive element undergoes significant pH dependent conformational changes. Specifically, it was demonstrated that the phylogenetically conserved A within the hairpin loop and an intra-loop C-G basepair are highly sensitive to changes in pH. The conserved C of the bulged loop does not significantly affect the pH dependent conformational changes of the hairpin loop. These studies have structural implications for an RNA-protein interaction that is critical to mammalian iron regulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 18:24:35