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Titolo:
Evaluation of buccal cell collection protocols for genetic susceptibility studies
Autore:
Moore, L; Wiencke, J; Eng, C; Zheng, SC; Smith, A;
Indirizzi:
Univ Calif Berkeley, Sch Publ Hlth, Berkeley, CA 94720 USA Univ Calif Berkeley Berkeley CA USA 94720 bl Hlth, Berkeley, CA 94720 USA Univ Calif San Francisco, Dept Epidemiol & Biostat, Lab Mol Epidemiol, SanFrancisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Calif San Francisco, Ctr Canc, San Francisco, CA 94143 USA Univ CalifSan Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA
Titolo Testata:
BIOMARKERS
fascicolo: 6, volume: 6, anno: 2001,
pagine: 448 - 454
SICI:
1354-750X(200111/12)6:6<448:EOBCCP>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
PCR AMPLIFICATION; DNA-POLYMERASE; MULTIPLEX PCR;
Keywords:
buccal cells; susceptibility; polymorphisms; molecular and genetic epidemiology;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
9
Recensione:
Indirizzi per estratti:
Indirizzo: Moore, L Univ Calif Berkeley, Sch Publ Hlth, 140 Warren Hall, Berkeley, CA94720 USA Univ Calif Berkeley 140 Warren Hall Berkeley CA USA 94720 720 USA
Citazione:
L. Moore et al., "Evaluation of buccal cell collection protocols for genetic susceptibility studies", BIOMARKERS, 6(6), 2001, pp. 448-454

Abstract

Buccal cells are increasingly used as a source of quality DNA to improve participation rates in molecular studies. Here, three buccal cell collectionprotocols were compared to determine factors affecting the yield of cells,total DNA per sample, and DNA yield per cell. In addition, kinetic quantitative polymerase chain reaction (PCR) (TaqMan(TM)) was used to quantify human DNA available for PCR. The method of collection used influenced the overall DNA yield per sample. The collection buffer used influenced the number of cells but not the overall DNA yield per sample. Repeated freezing and thawing did not affect overall DNA yield per sample, DNA yield per cell, or the total number of cells collected. Mouthwashes had the highest DNA yield per sample (20.8 mug) compared with cytobrush samples (1.9 mug from three cytobrushes) and tongue depressors (0.8 mug from three tongue depressors). However, mouthwash samples may contain significant non-human DNA and other contaminants that could interfere with some molecular studies. Spectrometry grossly overestimated the total DNA recovered from mouthwash samples compared with fluorometry or quantitative PCR.

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Documento generato il 21/09/20 alle ore 15:33:36