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Titolo:
Application of P450 reporter gene system (RGS) in the analysis of sediments near pulp and paper mills
Autore:
Jones, JM; Anderson, JW; Wiegel, JV; Tukey, RH;
Indirizzi:
Columbia Analyt Serv, Vista, CA 92083 USA Columbia Analyt Serv Vista CA USA 92083 Analyt Serv, Vista, CA 92083 USA Univ Calif Los Angeles, Dept Environm Sci & Engn, Los Angeles, CA USA UnivCalif Los Angeles Los Angeles CA USA ci & Engn, Los Angeles, CA USA Columbia Analyt Serv, Kelso, WA USA Columbia Analyt Serv Kelso WA USAColumbia Analyt Serv, Kelso, WA USA Univ Calif San Diego, Dept Pharmacol, San Diego, CA 92103 USA Univ Calif San Diego San Diego CA USA 92103 acol, San Diego, CA 92103 USA
Titolo Testata:
BIOMARKERS
fascicolo: 6, volume: 6, anno: 2001,
pagine: 406 - 416
SICI:
1354-750X(200111/12)6:6<406:AOPRGS>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYCYCLIC AROMATIC-HYDROCARBONS; TROUT ONCORHYNCHUS-MYKISS; HEPATOMA-CELL BIOASSAY; BLEACHED KRAFT PULP; ENVIRONMENTAL-SAMPLES; RESIN ACIDS; POLYCHLORINATED-BIPHENYLS; SECONDARY-TREATMENT; FISH BILE; INDUCTION;
Keywords:
pulp and paper; retene; CYP1A; dioxins;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Anderson, JW Columbia Analyt Serv, 1185 Pk Ctr Dr,Suite A, Vista, CA 92083USA Columbia Analyt Serv 1185 Pk Ctr Dr,Suite A Vista CA USA 92083
Citazione:
J.M. Jones et al., "Application of P450 reporter gene system (RGS) in the analysis of sediments near pulp and paper mills", BIOMARKERS, 6(6), 2001, pp. 406-416

Abstract

Cytochrome P4501A (CYP1A) induction in fish and other animals has been reported following exposure to pulp and paper mill effluent. Dioxins and furans as well as polycyclic aromatic hydrocarbons (PAH) are known inducers of CYP1A and have been found in sediments near pulp and paper mills. Retene (7-isopropyl-1-methylphenanthrene), an alkyl-substituted phenanthrene, has been recently associated with effluent and found to induce CYP1A in fish. Thisstudy utilized an in vitro assay, P450 Reporter Gene System (RGS), to assess the transcriptional activation of human CYP1A by retene after short (6 h) and long (16 h) exposures. Retene was as potent as benzo[ a] pyrene in inducing RGS, but was not as readily biotransformed by the cells. Extracts ofsediments collected near a pulp and paper mill were analysed, and RGS-derived toxic equivalencies (TEQ) were strongly correlated with Chemical TEQ analysis of dioxins and furans determined by EPA Method 8290 using high-resolution gas chromatography/mass spectrometry. RGS 6-h responses indicated thepresence of PAH in the extracts, which was confirmed by GC/MS analysis. Retene was detected at considerably higher concentrations than other PAH. These data support the use of the RGS assay to detect the presence of CYP1A-inducing compounds, including retene as well as dioxins and furans, in sediments near pulp mills.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 13:37:45