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Titolo:
Splice variants of human cathepsin L mRNA show different expression rates
Autore:
Abudula, A; Rommerskirch, W; Weber, E; Gunther, D; Wiederanders, B;
Indirizzi:
Klinikum Friedrich Schiller Univ, Inst Biochem 1, D-07743 Jena, Germany Klinikum Friedrich Schiller Univ Jena Germany D-07743 7743 Jena, Germany Univ Halle Wittenberg, Inst Physiol Chem, D-06114 Halle Saale, Germany Univ Halle Wittenberg Halle Saale Germany D-06114 4 Halle Saale, Germany
Titolo Testata:
BIOLOGICAL CHEMISTRY
fascicolo: 11, volume: 382, anno: 2001,
pagine: 1583 - 1591
SICI:
1431-6730(200111)382:11<1583:SVOHCL>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA; PROTEASE INHIBITORS; CELL CARCINOMA; BREAST-CANCER; LOCALIZATION; CYSTEINE; SEQUENCES; CLONING; ACID; TRANSFORMATION;
Keywords:
cathepsin L-ELISA; cis-acting element; kidney tumors; translation rate; 5 '-UTR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Wiederanders, B Klinikum Friedrich Schiller Univ, Inst Biochem 1, D-07743 Jena, Germany Klinikum Friedrich Schiller Univ Jena Germany D-07743 ny
Citazione:
A. Abudula et al., "Splice variants of human cathepsin L mRNA show different expression rates", BIOL CHEM, 382(11), 2001, pp. 1583-1591

Abstract

Human cathepsin L (hCATL) mRNA occurs in vivo in at least three splice variants. They differ in the length of exon 1, which comprises 278 nucleotides(hCATL-A), 188 nucleotides (hCATL-A2) and 132 nucleotides (hCATL-A3), respectively. We describe here the shortest variant for the first time. This form is predominant in all tissues and cells examined so far, including malignant tumors. We studied the expression rate of the three mRNA variants in order to explain why malignant kidney tumors show low cathepsin L activity despite of high mRNA levels. The variant hCATL-A3 showed the highest expression rate in vitro and in vivo. Based on these results, we suggest a cis-acting element on human cathepsin L mRNA which can be bound by a negative trans-acting regulator, thus leading to reduced expression rates.

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Documento generato il 04/04/20 alle ore 15:18:57