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Titolo:
Life without UCPI: mitochondrial, cellular and organismal characteristics of the UCPI-ablated mice
Autore:
Nedergaard, J; Golozoubova, V; Matthias, A; Shabalina, I; Ohba, KI; Ohlson, K; Jacobsson, A; Cannon, B;
Indirizzi:
Stockholm Univ, Arrhenius Labs F3, Wenner Gren Inst, SE-10691 Stockholm, Sweden Stockholm Univ Stockholm Sweden SE-10691 nst, SE-10691 Stockholm, Sweden
Titolo Testata:
BIOCHEMICAL SOCIETY TRANSACTIONS
, volume: 29, anno: 2001,
parte:, 6
pagine: 756 - 763
SICI:
0300-5127(2001)29:<756:LWUMCA>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
BROWN-ADIPOSE-TISSUE; DIET-INDUCED THERMOGENESIS; UNCOUPLING PROTEIN; FATTY-ACIDS; COLD; RATS;
Keywords:
brown adipose tissue; mitochondrial uncoupling; nonshivering thermogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Nedergaard, J Stockholm Univ, Arrhenius Labs F3, Wenner Gren Inst, SE-10691 Stockholm, Sweden Stockholm Univ Stockholm Sweden SE-10691 Stockholm, Sweden
Citazione:
J. Nedergaard et al., "Life without UCPI: mitochondrial, cellular and organismal characteristics of the UCPI-ablated mice", BIOCH SOC T, 29, 2001, pp. 756-763

Abstract

Mice devoid of the original uncoupling protein UCP1 have provided opportunities to delineate UCP1 function in a series of biochemical and physiological contexts. The isolated brown-fat mitochondria from such mice are fully coupled (without the addition of GDP), but still exhibit a depressed capacity for ATP synthesis. However, they only show a 2-fold decrease in sensitivity to the de-energizing effect of free fatty acids, compared with UCP1-containing mitochondria, whereas they possess a (UCP1-independent) 50-fold higher sensitivity than liver mitochondria; the fatty acid sensitivities in wildtype and UCP1-deficient mitochondria may, however, be of different natures. Despite the fact that brown-fat cells from UCP1-ablated mice cannot produce heat when stimulated by noradrenaline ('norepinephrine') or fatty acids,UCP1-ablated mice can be induced to tolerate extended cold exposure, but the heat then fully results from shivering thermogenesis. Recruitable or adaptive (by cold acclimation or adaptation to a cafeteria diet) adrenergically-stimulated thermogenesis does not exist in the UCP1-ablated animals, demonstrating the unique ability of UCP1 to mediate recruitable non-shivering thermogenesis. In addition to information on the function of UCP1, the UCP1-ablated mice can be used to gain information concerning the function of theUCP1 homologues. Thus whereas an uncoupling function of the UCP1 homologues cannot be excluded, UCP1-ablated animals clearly lack any ability, to recruit any UCP1 homologue to functionally replace the loss of thermogenesis resulting from UCP1. UCP1 (thermogenin) thus remains the only protein the activity of which can be recruited for the purpose of facultative thermogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 21:32:32