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Titolo:
Mechanisms of Ca2+ overload induced by extracellular H2O2 in quiescent isolated rat cardiomyocytes
Autore:
Gen, W; Tani, M; Takeshita, J; Ebihara, Y; Tamaki, K;
Indirizzi:
Keio Univ, Sch Med, Dept Geriatr, Shinjuku Ku, Tokyo 1608582, Japan Keio Univ Tokyo Japan 1608582 Geriatr, Shinjuku Ku, Tokyo 1608582, Japan
Titolo Testata:
BASIC RESEARCH IN CARDIOLOGY
fascicolo: 6, volume: 96, anno: 2001,
pagine: 623 - 629
SICI:
0300-8428(200111)96:6<623:MOCOIB>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARDIAC SARCOPLASMIC-RETICULUM; FREE-RADICAL GENERATION; RECOMBINANT SUPEROXIDE-DISMUTASE; VENTRICULAR MYOCYTES; HYDROGEN-PEROXIDE; ISCHEMIC MYOCARDIUM; REPERFUSION INJURY; NA+/CA2+ EXCHANGE; OXIDATIVE STRESS; CALCIUM-RELEASE;
Keywords:
free radicals; ischemia; reperfusion; sarcolemma; sarcoplasmic reticulum;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Tani, M Keio Univ, Sch Med, Dept Geriatr, Shinjuku Ku, 35 Shinanomachi, Tokyo 1608582, Japan Keio Univ 35 Shinanomachi Tokyo Japan 1608582 okyo 1608582, Japan
Citazione:
W. Gen et al., "Mechanisms of Ca2+ overload induced by extracellular H2O2 in quiescent isolated rat cardiomyocytes", BAS R CARD, 96(6), 2001, pp. 623-629

Abstract

Rat cardiomyocytes were exposed to H2O2 (1-100 mu mol/L) for 10 min with washout for 10 min. Intracellular Ca2+ concentration ([Ca2+](i)) was measured using fluo-3. [Ca2+](i) increased with 100 mu mol/L H2O2 and further increased during washout, causing irreversible contracture in one-half of the cells. The increase in [Ca2+](i) with 10 mu mol/L H2O2 was modest with few cells showing irreversible contracture and attenuated by caffeine, and [Ca-2](i) gradually decreased during washout and this decrease was accelerated by a calcium-free solution, while 1 mu mol/L H2O2 did not have any effects on [Ca2+](i) or cell viability. Ca2+ overload caused during exposure to 100mu mol/L H2O2 was attenuated by caffeine with improved cellular viability but not by chelerythrine, KB-R7943 or nifedipine. With 100 mu mol/L H2O2 calcium-free solution attenuated the increase during exposure and washout while KB-R7943 or chelerythrine partly attenuated further increase during washout but not improved cell viability, but chelerythrine did not have additional effect on calcium-free treatment. Catalase abolished the effects of H2O2. We concluded that the increased [Ca2+](i) during exposure to 100 mu mol/L H2O2 was caused both by release of Ca2+ from the intracellular store sites including the sarcoplasmic reticulum and by influx through route(s) otherthan the voltage-dependent Ca2+ channels or Na+/Ca2+ exchanger, although the Na+/Ca2+ exchanger or protein kinase C-mediated mechanism was partly responsible for a further increase during washout.

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Documento generato il 01/10/20 alle ore 16:08:58