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Titolo:
The beta m protein, a member of the X,K-ATPase beta-subunits family, is located intracellularly in pig skeletal muscle
Autore:
Pestov, NB; Korneenko, TV; Zhao, H; Adams, G; Kostina, MB; Shakhparonov, MI; Modyanov, NN;
Indirizzi:
Med Coll Ohio, Dept Pharmacol, Toledo, OH 43614 USA Med Coll Ohio Toledo OH USA 43614 o, Dept Pharmacol, Toledo, OH 43614 USA Russian Acad Sci, Shemyakin & Ovchinnikov Inst Bioorgan Chem, Moscow 117871, Russia Russian Acad Sci Moscow Russia 117871 oorgan Chem, Moscow 117871, Russia
Titolo Testata:
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
fascicolo: 1, volume: 396, anno: 2001,
pagine: 80 - 88
SICI:
0003-9861(200112)396:1<80:TBMPAM>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
TISSUE-SPECIFIC EXPRESSION; OUABAIN-SENSITIVE H,K-ATPASE; P-TYPE ATPASES; SARCOPLASMIC-RETICULUM; ENDOPLASMIC-RETICULUM; MEMBRANE-PROTEINS; ALPHA-SUBUNITS; PROTON PUMP; NA,K-ATPASE; NA+,K+-ATPASE;
Keywords:
protein expression; striated muscle; sarcoplasmic reticulum; P-type ATPase; Na,K-ATPase; ATP1B4;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Modyanov, NN Med Coll Ohio, Dept Pharmacol, POB 10008, Toledo, OH 43614 USA Med Coll Ohio POB 10008 Toledo OH USA 43614 edo, OH 43614 USA
Citazione:
N.B. Pestov et al., "The beta m protein, a member of the X,K-ATPase beta-subunits family, is located intracellularly in pig skeletal muscle", ARCH BIOCH, 396(1), 2001, pp. 80-88

Abstract

The sequence of the pig cDNA encoding the muscle-specific pm-protein, a member of the X,K-ATPase beta -subunits family, was determined. Two alternatively spliced transcripts encoding polypeptide chains of 355 and 351 residues were identified. The tissue specificity of expression of betam and other X,K-ATPase beta -subunit genes was studied by RT-PCR performed on 24 tissues from newborn pigs. The betam expression was shown to be highly tissue-specific, being detected at the highest level in skeletal muscle, at a lower level in heart, and at much lower level in skin. The betam transcripts are more abundant in the tissues from the newborn than adult. Immunoblotting anddeglycosylation shift assay indicated that skeletal muscle membranes of newborn pigs contain betam protein with an electrophoretic mobility and carbohydrate content very similar to that of human betam. Fractionation of membranes from both newborn and adult pig skeletal muscles by isopycnic centrifugation revealed that the majority of the betam protein is concentrated in the sarcoplasmic reticulum-containing fractions. This intracellular locationis a unique property that distinguishes the betam protein from other members of the YK-ATPase beta -subunit family. (C) 2001 Elsevier Science.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 11:33:27