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Titolo:
Hydrodynamic radii and lipid transfer in prostasome self-fusion
Autore:
Bordi, F; Cametti, C; De Luca, F; Carlini, E; Palmerini, CA; Arienti, G;
Indirizzi:
Sez Biochim, Dipartimento Med Interna & Sci Endocrine & Metabo, I-06122 Perugia, Italy Sez Biochim Perugia Italy I-06122 crine & Metabo, I-06122 Perugia, Italy Univ Roma La Sapienza, Dipartimento Fis, I-00185 Rome, Italy Univ Roma La Sapienza Rome Italy I-00185 imento Fis, I-00185 Rome, Italy INFM, Unita Roma 1, I-06122 Perugia, Italy INFM Perugia Italy I-06122INFM, Unita Roma 1, I-06122 Perugia, Italy Univ Perugia, Dipartimento Sci Biochim & Biotecnol Mol, I-06122 Perugia, Italy Univ Perugia Perugia Italy I-06122 Biotecnol Mol, I-06122 Perugia, Italy
Titolo Testata:
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
fascicolo: 1, volume: 396, anno: 2001,
pagine: 10 - 15
SICI:
0003-9861(200112)396:1<10:HRALTI>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXTRACELLULAR ORGANELLES PROSTASOMES; HUMAN SALIVARY-GLANDS; HUMAN SEMINAL PLASMA; MICROSOMAL-MEMBRANES; HUMAN-SPERMATOZOA; HUMAN-SEMEN; SPERM;
Keywords:
fusion; light scattering; membrane; prostasome; semen; human;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Arienti, G Sez Biochim, Dipartimento Med Interna & Sci Endocrine & Metabo,Via Giochetto, I-06122 Perugia, Italy Sez Biochim Via Giochetto Perugia Italy I-06122 Perugia, Italy
Citazione:
F. Bordi et al., "Hydrodynamic radii and lipid transfer in prostasome self-fusion", ARCH BIOCH, 396(1), 2001, pp. 10-15

Abstract

The characteristics of human prostasomal vesicles have been investigated by three methods, namely, dynamic light scattering, transfer of a lipophylicfluorescent dye (R-18), and electron microscope appearance. The vesicle preparations were stable for a long time and their diameters were in the range of 200 nm. The exposure to acidic pH values (about 5) increased both particle radii and the transfer of R-18. The microscopic appearance was also affected by the pH value. We infer that these changes are due to a self-fusion of prostasome vesicles; this fusion is protein-dependent since various methods used by us and able to affect the protein component of membranes (boiling, extraction of lipid and liposome preparation, treatment with pronase)all abolished the effect seen at pH 5 on intact particles. (C) 2001 Elsevier Science.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 15:10:11