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Titolo:
Characterization of neutralization epitopes of simian immunodeficiency virus (SIV) recognized by rhesus monoclonal antibodies derived from monkeys infected with an attenuated SIV strain
Autore:
Cole, KS; Alvarez, M; Elliott, DH; Lam, H; Martin, E; Chau, T; Micken, K; Rowles, JL; Clements, JE; Murphey-Corb, M; Montelaro, RC; Robinson, JE;
Indirizzi:
Tulane Univ, Sch Med, Dept Pediat, New Orleans, LA 70112 USA Tulane Univ New Orleans LA USA 70112 pt Pediat, New Orleans, LA 70112 USA Univ Pittsburgh, Sch Med, Dept Mol Genet & Biochem, Pittsburgh, PA 15261 USA Univ Pittsburgh Pittsburgh PA USA 15261 Biochem, Pittsburgh, PA 15261 USA Johns Hopkins Univ, Sch Med, Dept Comparat Med, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 at Med, Baltimore, MD 21205 USA Johns Hopkins Univ, Sch Med, Dept Mol Biol & Genet, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 Genet, Baltimore, MD 21205 USA
Titolo Testata:
VIROLOGY
fascicolo: 1, volume: 290, anno: 2001,
pagine: 59 - 73
SICI:
0042-6822(20011110)290:1<59:CONEOS>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXTERNAL ENVELOPE GLYCOPROTEIN; INTRACHAIN DISULFIDE BONDS; PASSIVE-IMMUNIZATION; MOLECULAR CLONES; MACAQUES; TYPE-1; IDENTIFICATION; GP120; PROTEIN; CHALLENGE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
68
Recensione:
Indirizzi per estratti:
Indirizzo: Robinson, JE Tulane Univ, Sch Med, Dept Pediat, 1430 Tulane Ave, New Orleans, LA 70112 USA Tulane Univ 1430 Tulane Ave New Orleans LA USA 70112 0112 USA
Citazione:
K.S. Cole et al., "Characterization of neutralization epitopes of simian immunodeficiency virus (SIV) recognized by rhesus monoclonal antibodies derived from monkeys infected with an attenuated SIV strain", VIROLOGY, 290(1), 2001, pp. 59-73

Abstract

A major limitation in the simian immunodeficiency virus (SIV) system has been the lack of reagents with which to identify the antigenic determinants that are responsible for eliciting neutralizing antibody responses in macaques infected with attenuated SIV. Most of our information on SIV neutralization determinants has come from studies with murine monoclonal antibodies (MAbs) produced in response to purified or recombinant SIV envelope proteinsor intact SIV-infected cells for relatively short periods of time. While these studies provide some basic information on the potential immunogenic determinants of SIV envelope proteins, it is unclear whether these murine MAbs identify epitopes relevant to antibody responses elicited in monkeys during infection with either wild-type or attenuated SIV strains. To accomplishmaximum biological relevance, we developed a reliable method for the production of rhesus monoclonal antibodies. In the present study, we report on the production and characterization of a unique panel of monoclonal antibodies derived from four individual monkeys inoculated with SIV/17E-CL as an attenuated virus strain at a time when protective immunity from pathogenic challenge was evident. Results from these studies identified at least nine binding domains on the surface envelope glycoprotein; these included linear determinants in the V1, V2, cysteine loop (analogous to the V3 loop in humanimmunodeficiency virus type 1), and C5 regions, as well as conformational epitopes represented by antibodies that bind the C-terminal half of gp120 and those sensitive to defined mutations in the V4 region. More importantly,three groups of antibodies that recognize closely related, conformational epitopes exhibited potent neutralizing activity against the vaccine strain. Identification of the epitopes recognized by these neutralizing antibodieswill provide insight into the antigenic determinants responsible for eliciting neutralizing antibodies in vivo that can be used in the design of effective vaccine strategies. (C) 2001 Academic Press.

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Documento generato il 28/03/20 alle ore 23:09:54