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Titolo:
Transgenic tobacco plants expressing the maize Cat2 gene have altered catalase levels that affect plant-pathogen interactions and resistance to oxidative stress
Autore:
Polidoros, AN; Mylona, PV; Scandalios, JG;
Indirizzi:
N Carolina State Univ, Dept Genet, Raleigh, NC 27695 USA N Carolina State Univ Raleigh NC USA 27695 t Genet, Raleigh, NC 27695 USA
Titolo Testata:
TRANSGENIC RESEARCH
fascicolo: 6, volume: 10, anno: 2001,
pagine: 555 - 569
SICI:
0962-8819(200112)10:6<555:TTPETM>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYSTEMIC ACQUIRED-RESISTANCE; MANGANESE SUPEROXIDE-DISMUTASE; SALICYLIC-ACID; DISEASE RESISTANCE; HYDROGEN-PEROXIDE; DEFENSE RESPONSES; ACTIVATION; INDUCTION; SIGNAL; H2O2;
Keywords:
catalase; disease resistance; gene silencing; plant-pathogen interactions; reactive oxygen;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Scandalios, JG N Carolina State Univ, Dept Genet, Box 7614, Raleigh, NC 27695 USA N Carolina State Univ Box 7614 Raleigh NC USA 27695 695 USA
Citazione:
A.N. Polidoros et al., "Transgenic tobacco plants expressing the maize Cat2 gene have altered catalase levels that affect plant-pathogen interactions and resistance to oxidative stress", TRANSGEN RE, 10(6), 2001, pp. 555-569

Abstract

Transgenic tobacco genotypes expressing the maize Cat2 gene were developedwith altered catalase (CAT) levels that resulted in a moderate increase ofCAT activity in two transgenic lines. Bacterial infection, with a pathogenthat does not share homology with the transgene, caused local and systemicdown-regulation of the steady state mRNA levels of the 35S-driven transgene in a manner resembling post-transcriptional gene silencing (PTGS). Phenotypic symptoms of hypersensitive response (HR) and systemic acquired resistance (SAR) were similar in control SR1 and the transgenic genotypes. Induction of hin1, used as a molecular marker of plant responses to invading bacteria, displayed a similar pattern between control and transgenic lines, but some variation in the levels of expression was observed. The major difference was recorded in the ability of the plants to restrict bacterial growth during HR. All transgenic lines were more sensitive than control SR1, with two lines exhibiting a significantly reduced capacity to inhibit bacterial growth. This is consistent with the putative enhanced capacity of transgeniclines containing the maize Cat2 gene to more effectively remove H2O2, which may act as a direct antimicrobial agent. Steady state mRNA levels of PR-1and PR-5 varied among the genotypes, possibly indicating differences in strength of the SAR signal. Transgenic line 2, which was the most sensitive during HR, was most effective in restricting bacterial growth during SAR. This indicates that a reverse correlation might exist between the severity ofinfection during HR and the ability to inhibit bacterial growth during SAR. Growth under high light conditions affected plant-pathogen interactions in control SR1, as well as in transgenic line 8. Early induction and higher expression of PR-1 and PR-5 was detected in both SR1 and line 8 in high light-grown plants as compared with their low light-grown counterparts. Our data indicate that growth under high light conditions can predispose plants to better resist pathogen attack, and may amplify local and systemic defensesignals. Finally, one transgenic line, which exhibited 1.3-fold higher average CAT activity in comparison with the untransformed SR1 control, suffered significantly less methyl viologen (MV) damage than untransformed controlplants at moderate and high MV concentrations.

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Documento generato il 03/04/20 alle ore 10:59:43