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Titolo:
Cord blood G(0) CD34(+) cells have a thousand-fold higher capacity for generating progenitors in vitro than G(1) CD34(+) cells
Autore:
Summers, YJ; Heyworth, CM; de Wynter, EA; Chang, J; Testa, NG;
Indirizzi:
Paterson Inst Canc Res, CRC, Expt Haematol Grp, Manchester M20 4BX, Lancs,England Paterson Inst Canc Res Manchester Lancs England M20 4BX BX, Lancs,England
Titolo Testata:
STEM CELLS
fascicolo: 6, volume: 19, anno: 2001,
pagine: 505 - 513
SICI:
1066-5099(2001)19:6<505:CBGCCH>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC STEM-CELLS; EX-VIVO EXPANSION; COMBINED IMMUNODEFICIENT MICE; MOBILIZED PERIPHERAL-BLOOD; SELF-RENEWAL; FUNCTIONAL-HETEROGENEITY; REPOPULATING CAPACITY; FLT3/FLK-2 LIGAND; CYCLE STATUS; ENGRAFTMENT;
Keywords:
CD34; cord blood; ex vivo expansion; cell cycle; serum-free; G(0);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Summers, YJ Paterson Inst Canc Res, CRC, Expt Haematol Grp, Manchester M204BX, Lancs,England Paterson Inst Canc Res Manchester Lancs England M20 4BX gland
Citazione:
Y.J. Summers et al., "Cord blood G(0) CD34(+) cells have a thousand-fold higher capacity for generating progenitors in vitro than G(1) CD34(+) cells", STEM CELLS, 19(6), 2001, pp. 505-513

Abstract

We examined the functional differences between G(0) and G(1) cord blood CD34(+) cells for up to 24 weeks in serum-free suspension culture, containingFlt-3 ligand, thrombopoietin and stem cell factor. By week 24, there is more than a 1,000-fold difference in granulocyte, macrophage-colony-forming cells (GM-CFC) cumulative production between the two populations, with cultures initiated from G(0) demonstrating an amplification of 1.1 x 10(5)-1.8 x10(6) of GM-CFC compared to 45-2.7 x 10(3) for the G(1) cells. Cells from the initial G(0) population are able to produce about 250-fold higher numbers of BFU-E than those from G(1) which translates to 3 x 10(3)-1.1 x 10(5)-fold expansion and 25-390-fold expansion for G(0) and G(1), respectively. This amplification of the progenitor cells is reflected in finding that a greater proportion of the progeny of the G(0) population are CD34(+), resulting in a 600-fold expansion of CD34(+) cells at week 8. As in other in vitrosystems, total cell expansion is less discriminatory of stem cell behaviorthan progenitor cells, and there is no significant difference in total cell numbers between G(0) and G(1) cultures with a mean fold expansion of 2 x 10(7) at 24 weeks.

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Documento generato il 29/03/20 alle ore 00:08:55