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Titolo:
Assembly and use of a broadly applicable neural cDNA microarray
Autore:
Barrett, T; Cheadle, C; Wood, WH; Teichberg, D; Donovan, DM; Freed, WJ; Becker, KG; Vawter, MP;
Indirizzi:
NIA, DNA Array Unit, Res Resources Branch, NIH, Baltimore, MD 21224 USA NIA Baltimore MD USA 21224 Resources Branch, NIH, Baltimore, MD 21224 USA NIA, Transgen & Knockout Facil Sect, NIH, Baltimore, MD 21224 USA NIA Baltimore MD USA 21224 ckout Facil Sect, NIH, Baltimore, MD 21224 USA NIDA, Cellular Neurobiol Branch, Baltimore, MD 21224 USA NIDA Baltimore MD USA 21224 lar Neurobiol Branch, Baltimore, MD 21224 USA
Titolo Testata:
RESTORATIVE NEUROLOGY AND NEUROSCIENCE
fascicolo: 2-3, volume: 18, anno: 2001,
pagine: 127 - 135
SICI:
0922-6028(2001)18:2-3<127:AAUOAB>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-EXPRESSION PATTERNS; BRAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Barrett, T NIA, DNA Array Unit, Res Resources Branch, NIH, Rm 4-D16,5600 Nathan ShockDr, Baltimore, MD 21224 USA NIA Rm 4-D16,5600 Nathan Shock Dr Baltimore MD USA 21224 24 USA
Citazione:
T. Barrett et al., "Assembly and use of a broadly applicable neural cDNA microarray", REST NEUROL, 18(2-3), 2001, pp. 127-135

Abstract

cDNA microarrays provide an efficient method to analyze gene expression patterns in thousands of genes in parallel. In some cases, large unfocused collections of cDNAs have been used in hybridization studies, in others smalllogically defined collections of tissue specific arrays have been used. Here we describe the bioinformatic selection of 1152 named human cDNAs specifically designed for neuroscience applications, arrayed on nylon membranes at high density. cDNAs were chosen which represent all the major cellular types of the brain including; neurons, astrocytes, microglia, and oligodendrocytes. Gene families chosen include cell type specific markers, ion-channels, transporters, receptors, and cell adhesion molecules among many others. These arrays were used with region specific samples from human brain to determine mRNA expression profiles for each region. Used with (33)-P labeled complex probes, this is a low cost, highly sensitive approach for the investigator to focus on tissue specific genes of interest where samples of limiting amounts of RNA are used. This selected set of brain-relevant cDNAs should be widely useful in the analysis of gene expression patterns from brain tissues as well as neural cell lines.

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Documento generato il 12/07/20 alle ore 12:32:07