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Titolo:
Gene expression profile in early stage of retinoic acid-induced differentiation of human SH-SY5Y neuroblastoma cells
Autore:
Truckenmiller, ME; Vawter, MP; Cheadle, C; Coggiano, M; Donovan, DM; Freed, WJ; Becker, KG;
Indirizzi:
NIDA, Cellular Neurobiol Res Branch, IRP, NIH, Baltimore, MD 21224 USA NIDA Baltimore MD USA 21224 Res Branch, IRP, NIH, Baltimore, MD 21224 USA NIA, DNA Array Unit, NIH, Baltimore, MD 21224 USA NIA Baltimore MD USA 21224 , DNA Array Unit, NIH, Baltimore, MD 21224 USA Univ Calif Irvine, Irvine, CA 92697 USA Univ Calif Irvine Irvine CA USA 92697 Calif Irvine, Irvine, CA 92697 USA
Titolo Testata:
RESTORATIVE NEUROLOGY AND NEUROSCIENCE
fascicolo: 2-3, volume: 18, anno: 2001,
pagine: 67 - 80
SICI:
0922-6028(2001)18:2-3<67:GEPIES>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEURO-BLASTOMA CELLS; NERVE GROWTH-FACTOR; OUTGROWTH; APOPTOSIS; INDUCTION; CULTURE; DISPLAY; RNA;
Keywords:
microarray; retinoic acid; differentiation; neurofilament; profilin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Truckenmiller, ME NIDA, Cellular Neurobiol Res Branch, IRP, NIH, 5500 Nathan Shock Dr, Baltimore, MD 21224 USA NIDA 5500 Nathan Shock Dr Baltimore MDUSA 21224 224 USA
Citazione:
M.E. Truckenmiller et al., "Gene expression profile in early stage of retinoic acid-induced differentiation of human SH-SY5Y neuroblastoma cells", REST NEUROL, 18(2-3), 2001, pp. 67-80

Abstract

Purpose: The human SH-SY5Y cell line is an established model for retinoic acid (RA)-induced neural differentiation. We employed a broad human 15K microarray (15,000 genes) and focused Neuroarray (1152 genes) to examine changes in gene expression early in the process of differentiation (6 hr), before morphology or growth changes are observed. Methods: P-33-labeled cDNA probes prepared from RNA extracts of RA-treatedand control cultures were hybridized to array membranes, and levels of expression were quantified and compared. Results: In the 15K array, 19 % of the genes were decreased (0.4 % were named genes and the remainder were expressed sequence tags (ESTs) or unknowns), and 9 % were increased (4.2 % named genes). In the Neuroarray, 3 % were decreased and 8 % were increased. Conclusions: Summary gene profiles are presented, which include transcription factors, genes associated with cell cycle, cell shape, neurotransmission, intermediary filaments, and others. The prevalence of down-regulated genes in the broad 15K array and up-regulated genes in the neuro-focused arraysuggests a pattern shift in gene expression associated with differentiation. The predominance of ESTs among the down-regulated genes indicates a great number of as-yet-unidentified genes are repressed in early stage neural differentiation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 21:25:41