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Titolo:
Isolation and immunolocalization of a Pinus nigra lectin (PNL) during interaction with the necrotrophs - Heterobasidion annosum and Fusarium avenaceum
Autore:
Nahalkova, J; Asiegbu, FO; Daniel, G; Hrib, J; Vookova, B; Pribulova, B; Gemeiner, P;
Indirizzi:
Swedish Univ Agr Sci, Dept Forest Mycol & Pathol, S-75007 Uppsala, Sweden Swedish Univ Agr Sci Uppsala Sweden S-75007 hol, S-75007 Uppsala, Sweden Slovak Acad Sci, Inst Chem, SK-84238 Bratislava, Slovakia Slovak Acad SciBratislava Slovakia SK-84238 -84238 Bratislava, Slovakia Swedish Univ Agr Sci, Dept Wood Sci, S-75007 Uppsala, Sweden Swedish Univ Agr Sci Uppsala Sweden S-75007 Sci, S-75007 Uppsala, Sweden Slovak Acad Sci, Inst Plant Genet, SK-95007 Nitra, Slovakia Slovak Acad Sci Nitra Slovakia SK-95007 Genet, SK-95007 Nitra, Slovakia
Titolo Testata:
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
fascicolo: 3, volume: 59, anno: 2001,
pagine: 153 - 163
SICI:
0885-5765(200109)59:3<153:IAIOAP>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
CULTURED RICE CELLS; N-ACETYLCHITOOLIGOSACCHARIDE ELICITOR; WHEAT-GERM-AGGLUTININ; NORWAY SPRUCE; CYTOCHEMICAL-LOCALIZATION; BINDING-PROTEIN; NYLON MEMBRANES; SEEDLING ROOTS; FUNGAL GROWTH; PICEA-ABIES;
Keywords:
Pinus nigra; Pinus sylvestris; Picea abies; Heterobasidion annosum; Fusarium avenaceum; N-acetyl-beta-D-glucosamine; recognition; fungi; root rot;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Nahalkova, J Swedish Univ Agr Sci, Dept Forest Mycol & Pathol, Box 7026, S-75007 Uppsala, Sweden Swedish Univ Agr Sci Box 7026 Uppsala Sweden S-75007 Sweden
Citazione:
J. Nahalkova et al., "Isolation and immunolocalization of a Pinus nigra lectin (PNL) during interaction with the necrotrophs - Heterobasidion annosum and Fusarium avenaceum", PHYSL MOL P, 59(3), 2001, pp. 153-163

Abstract

Pinus nigra ARN. lectin (PNL) was isolated from protein bodies of Europeanblack pine seeds by affinity chromatography of protein bodies extract on GIcNAc-C-glycosylated Spheron 300 beads. In vitro tests confirmed that PNL has the ability to agglutinate rat erythrocytes, GIcNAc-C-glycosylated HEMA BIO 1000 microbeads and Trichoderma viride spores. Western blot immuno-assays using antibodies against PNL antigen demonstrated specificity of the antisera. Immuno dot assays with polyclonal antibody against the purified PNL exhibited strong cross reaction to both Scots pine and Norway spruce seed extracts but not to fungal cell wall extracts from either Heterobasidion annosum or Fusarium avenaceum or Phlebiopsis gigantea. The indirect immunofluorescence assay showed a strong interaction of PNL with hyphal materials of the necrotrophs (H. annosum and F avenaceum). Results from immunolocalization experiments suggest that PNL is not degraded during seed germination anddevelopment since it was localized in all parts of juvenile pine seedlings. The protein was mainly observed on the cytoplasmic membranes and on the primary cell walls. In infected seedlings, a strong labelling of hyphal materials with PNL antisera was recorded only at the early stages of infection but not at the later stages of hyphal invasion. At advanced stages of the infection, most of the fungal hyphae were covered by host phenolic-like substances which probably masked the interaction of the antiserum with pathogencell wall materials; hence the low labelling. The potential function of PNL as a recognition molecule during interaction of conifer trees with necrotrophic parasites is discussed. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 07:44:24