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Titolo:
Suppression of conditioned fear by administration of CCKB receptor antisense oligodeoxynucleotide into the lateral ventricle
Autore:
Tsutsumi, T; Akiyoshi, J; Hikichi, T; Kiyota, A; Kohno, Y; Katsuragi, S; Yamamoto, Y; Isogawa, K; Nagayama, H;
Indirizzi:
Oita Med Univ, Dept Neuropsychiat, Oita 8795593, Japan Oita Med Univ Oita Japan 8795593 Dept Neuropsychiat, Oita 8795593, Japan
Titolo Testata:
PHARMACOPSYCHIATRY
fascicolo: 6, volume: 34, anno: 2001,
pagine: 232 - 237
SICI:
0176-3679(200111)34:6<232:SOCFBA>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHOLECYSTOKININ-B RECEPTOR; PANIC DISORDER; CALCIUM MOBILIZATION; PHARMACOLOGICAL EVALUATION; RAT-BRAIN; T-CELLS; ANTAGONIST; ANXIETY; STRESS; TETRAPEPTIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Akiyoshi, J Oita Med Univ, Dept Neuropsychiat, Hasama Machi, Oita 8795593,Japan Oita Med Univ Hasama Machi Oita Japan 8795593 8795593, Japan
Citazione:
T. Tsutsumi et al., "Suppression of conditioned fear by administration of CCKB receptor antisense oligodeoxynucleotide into the lateral ventricle", PHARMACOPS, 34(6), 2001, pp. 232-237

Abstract

We investigated the role of CCK in the development of anxiety by determining whether CCKB receptor antisense suppressed intracellular Ca2+ concentration in vitro or suppressed conditioned fear stress in vivo. First, for the in vitro studies, we used rat pituitary tumor GH3 cells since these cells have CCKB receptors. GH3 cells were, stimulated by 10 muM CCK-4; intracellular Ca2+ concentration was measured. The CCKB receptor antisense at 1 or 10 muM reduced the subsequent response to 10 muM CCK-4 in a time-dependent manner. Second, for the in vivo studies, the CCKB receptor antisense, sense, random sense, or saline was infused at a constant rate for 6 days into rat lateral ventricles via mini-osmotic pumps. Individual rats were then subjected to 30 min of inescapable electric footshock in a chamber with a grid floor. Twenty-four hours later, the rat was again placed in the chamber and observed for 5 min without shocks. This study showed. that CCKB receptor antisense significantly suppressed intracellular Ca2+ concentration in GH3 cells and significantly reduced freezing behavior in rats, indicating that the CCKB receptor plays an important role in anxiety.

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Documento generato il 05/12/20 alle ore 08:14:35