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Titolo:
Identification of a novel voltage-gated Na+ channel rNa(v)1.5a in the rat hippocampal progenitor stem cell line HiB5
Autore:
Korsgaard, MPG; Christophersen, P; Ahring, PK; Olesen, SP;
Indirizzi:
NeuroSearch AS, DK-2750 Ballerup, Denmark NeuroSearch AS Ballerup Denmark DK-2750 ch AS, DK-2750 Ballerup, Denmark Panum Inst, DK-2200 Copenhagen N, Denmark Panum Inst Copenhagen Denmark N anum Inst, DK-2200 Copenhagen N, Denmark
Titolo Testata:
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
fascicolo: 1, volume: 443, anno: 2001,
pagine: 18 - 30
SICI:
0031-6768(200110)443:1<18:IOANVN>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPINAL-CORD ASTROCYTES; DORSAL-ROOT GANGLIA; SODIUM-CHANNELS; MESSENGER-RNA; MOLECULAR DETERMINANTS; SLOW INACTIVATION; SENSORY NEURONS; ALPHA-SUBUNIT; ION CHANNELS; GLIAL-CELLS;
Keywords:
Na+ channel; rNa(v)1.5a; lamotrigine; sipatrigine; state-dependent; HiB5; patch-clamp; tetrodotoxin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Korsgaard, MPG NeuroSearch AS, Pederstrupvej 93, DK-2750 Ballerup, DenmarkNeuroSearch AS Pederstrupvej 93 Ballerup Denmark DK-2750 k
Citazione:
M.P.G. Korsgaard et al., "Identification of a novel voltage-gated Na+ channel rNa(v)1.5a in the rat hippocampal progenitor stem cell line HiB5", PFLUG ARCH, 443(1), 2001, pp. 18-30

Abstract

A conditionally immortalised cell line, HiB5, derived from embryonic hippocampal precursor cells expressed a voltage-gated Nat channel with electrophysiological characteristics shifted to more negative voltages and a lower sensitivity to tetrodotoxin [concentration required for half-maximal inhibition (IC50) 0.9 muM] compared with endogenous; neuronal Na+ channels. The channel activation and steady-state inactivation occurred at very negative potentials with the threshold for activation at -55 mV and half-maximal inactivation at -78.7 mV. The channel was blocked by lamotrigine and sipatriginevoltage and state dependently, with potencies 5-20 times higher (IC50 12 and 1.8 muM at -80 mV respectively) than the corresponding block of endogenous Ne channels from neurones and cloned rNa(v)1.2a (rBIIA) alpha -subunits. Both compounds slowed the channel's recovery from inactivation. Whereas lamotrigine and sipatrigine had similar effects on the fast inactivated state, the effect of sipatrigine on the slow inactivation state was more pronounced, rendering this compound overall the more effective. The molecular subtype mainly expressed by HiB5 cells was identified using RT-PCR and was a novel splice variant of rNa(v)1.5 (rNa(v)1.5a). It differs from the known rNa(v)1.5 version in that it lacks 53 amino acids in the intracellular loop between domains H and III. rNa(v)1.5a was also detected in mRNA derived from rat whole brain.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 20:09:47