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Titolo:
Effect of increased lung expansion on surfactant protein mRNA levels in lambs
Autore:
Lines, AL; Davey, MG; Harding, R; Hooper, SB;
Indirizzi:
Monash Univ, Dept Physiol, Clayton, Vic 3800, Australia Monash Univ Clayton Vic Australia 3800 siol, Clayton, Vic 3800, Australia
Titolo Testata:
PEDIATRIC RESEARCH
fascicolo: 6, volume: 50, anno: 2001,
pagine: 720 - 725
SICI:
0031-3998(200112)50:6<720:EOILEO>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
FETAL SHEEP; TRACHEAL OBSTRUCTION; RESPIRATORY-FUNCTION; GENE-EXPRESSION; RAT LUNG; GROWTH; VENTILATION; PRESSURE; HYPERVENTILATION; DISTENSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Hooper, SB Monash Univ, Dept Physiol, Clayton, Vic 3800, Australia Monash Univ Clayton Vic Australia 3800 on, Vic 3800, Australia
Citazione:
A.L. Lines et al., "Effect of increased lung expansion on surfactant protein mRNA levels in lambs", PEDIAT RES, 50(6), 2001, pp. 720-725

Abstract

Increased fetal lung expansion profoundly inhibits surfactant protein geneexpression and stimulates cellular proliferation in the fetal lung. Our aim was to determine whether increased expansion of the lung after birth, by the application of a continuous positive airway pressure (CPAP) for 12 h, inhibits surfactant protein gene expression and stimulates cell division in lambs. Two week-old lambs were randomly divided into 2 groups (n = 5 for each), sedated, and exposed to either no CPAP (controls) or 10cmH(2)O Of CPAPduring a 12-hour treatment period. After 2 h of the treatment, H-3-thymidine was administered to each lamb (iv) to measure pulmonary DNA synthesis rates over the following 10 h of treatment. To assess the increase in lung expansion, functional residual capacity (FRC) was measured before the start of the treatment period and again at 6 and 12 h. Compared with control lambs, a CPAP of 10 cmH(2)O increased FRC from 26.8 +/- 3.8 mL/kg to 62.9 +/- 19.7 mL/kg at 6 h and it remained elevated at 12 h (56.2 +/- 5.7 mL/kg). Despite this large increase in end expiratory lung volume (FRC), the mRNA levels for SP-A, SP-B, and SP-C and DNA synthesis rates in lung tissue were not altered. The results of this study indicate that, in contrast to the fetus,an increase in end expiratory lung volume of similar to 100% does not affect surfactant protein gene expression or pulmonary DNA synthesis rates in 2week old lambs. Thus, the response of the lung to increases in lung expansion varies markedly before and after birth.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 17:35:07