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Titolo:
Microtubule-associated protein 2 phosphorylation is decreased in the humanepileptic temporal lobe cortex
Autore:
Sanchez, C; Arellano, J; Rodriguez-Sanchez, P; Avila, J; DeFelipe, J; Diez-Guerra, FJ;
Indirizzi:
Univ Autonoma Madrid, Fac Ciencias, Dept Biol Mol, Ctr Biol Mol Severo Ochoa, E-28049 Madrid, Spain Univ Autonoma Madrid Madrid Spain E-28049 o Ochoa, E-28049 Madrid, Spain CSIC, Inst Cajal, E-28002 Madrid, Spain CSIC Madrid Spain E-28002CSIC, Inst Cajal, E-28002 Madrid, Spain
Titolo Testata:
NEUROSCIENCE
fascicolo: 1, volume: 107, anno: 2001,
pagine: 25 - 33
SICI:
0306-4522(2001)107:1<25:MP2PID>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLYCOGEN-SYNTHASE KINASE-3-BETA; NMDA-GLUTAMATE RECEPTORS; RAT HIPPOCAMPAL-NEURONS; LONG-TERM POTENTIATION; MAP2 PHOSPHORYLATION; CATALYZED PHOSPHORYLATION; RAPID DEPHOSPHORYLATION; SYNAPTIC PLASTICITY; BRAIN-DEVELOPMENT; DENDRITIC SPINES;
Keywords:
epilepsy; degeneration; cytoarchitecture; cytoskeleton; electrocorticogram;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
68
Recensione:
Indirizzi per estratti:
Indirizzo: Diez-Guerra, FJ Univ Autonoma Madrid, Fac Ciencias, Dept Biol Mol, Ctr Biol Mol Severo Ochoa, E-28049 Madrid, Spain Univ Autonoma Madrid Madrid Spain E-28049 Madrid, Spain
Citazione:
C. Sanchez et al., "Microtubule-associated protein 2 phosphorylation is decreased in the humanepileptic temporal lobe cortex", NEUROSCIENC, 107(1), 2001, pp. 25-33

Abstract

Microtubule-associated protein 2 (MAP2) is an abundant component of the neuronal cytoskeleton whose function is related to the outgrowth and stability of neuronal processes, to synaptic plasticity and neuronal cell death. Wehave sought to study whether abnormal patterns of neuronal activity which are characteristic of epileptic patients are associated to alterations of MAP2 phosphorylation. An antibody (305) that selectively recognizes a phosphorylated epitope in a proline-rich region of the MAP2 molecule has been used to analyze neocortical biopsy samples from temporal lobe epileptic patients, whose electrocorticogram activity had been previously monitored. Immunoblot analysis showed that samples with greater spiking activity displayed significantly diminished MAP2 phosphorylation. Immunocytochemical analysis revealed the occurrence of discrete areas in the neocortex with highly decreased or no immunostaining for antibody 305, which showed a clear, although non-significant, tendency to appear more frequently in areas with greater spiking activity. To further support an association between epileptiform activity and MAP2 dephosphorylation an experimental model of epileptiform activity in cultures of rat hippocampal neurons was used. Neurons were culturedduring 15 days in the presence of kynurenic acid. an antagonist of glutamate receptors. At this time, kynurenic acid was removed from the culture medium and neurons developed seizure-like activity. Using antibody 305, we found a decrease of MAP2 phosphorylation that was already visible after 15 minof kynurenic acid withdrawal. We therefore propose that MAP2 phosphorylation is decreased in the neocortex of epileptic patients and that this decrease is a likely consequence of seizure activity. Also, MAP-2 dephosphorylation may lead to alterations of the neuronal cytoskeleton and eventually to neuronal damage and loss, whichis typical of epileptic patients. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.

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Documento generato il 05/04/20 alle ore 05:28:45